Objective
The proposed project is dedicated to the development of a novel approach, called FLPFLEX, to manipulate gene expression in the mouse, for dissecting complex genetic pathways and for providing more accurate models of human disease. By engaging the cell's ow n transcriptional regulatory circuits to capture novel expression patterns, FLPFLEX cassette exhange allows multiple rapid manipulations in a single genomic locus, providing powerful and adaptable tools for designing increasingly complex genetic models of human physiology and pathology. In Module 1 will include development of readout vector technologies and establishment of the FLPFLEX cell library. We will develop flexible genomic insertion cassettes carrying modifications to allow recombinase-mediated cas sette exchange of effector genes of interest. The FLPFLEX vector carrying a multifunctional tag will be randomly incorporated into the genome of embryonic stem (ES) cells. Since a majority of all mouse genes are expressed in ES cells, multiple ES clones ca rrying an expressed FLPFLEX tag can be readily selected. We will sequence and identify 10,000 FLPFLEX cassette integration sites using mouse genomic information. Predicted expression patterns of the captured gene locus will be verified by manual and automa ted in situ hybridization of adult tissues. In Module 2, we will select 50 FLPFLEX clones for further characterization and focused studies in vivo. We will introduce different effector genes via cassette exchange in selected FLPFLEX cell clones. Germ line transmission of these exchange cassettes in mice will yield novel patterns for conditional effector genes such as Cre recombinase, or allelic exchange of individual mutated cDNAs of disease genes. The project is strategically designed to provide an efficie nt mechanism for generating, characterising and disseminating these models. Information and reagents generated will be disseminated on a publicly accessible database. '
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences databases
- medical and health sciences basic medicine pathology
- medical and health sciences basic medicine physiology
- natural sciences biological sciences genetics genomes
You need to log in or register to use this function
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2003-LIFESCIHEALTH-I
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
HEIDELBERG
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.