Objective
The proposed project centres on so far neglected animal-microbe interactions in freshwater sediment that are expected to greatly influence denitrification rates and N2O emission to the atmosphere. We hypothesise that the guts of sediment-dwelling detritivores constitute "mobile microsites" in which bacterial denitrification will be induced by anoxia and high concentrations ofdissolved organic carbon. Over time, the inoculation of sediment with actively denitrifying bacteria via egested feces may favour the development of a structurally and functionally distinct denitrifying community.
In a fully integrated approach using the contemporary techniques of Microbial Ecology we aim at:
(i) characterising the gut microenvironment of the abundant detritivore Chironomus r iparius (Insecta: Diptera) withmicrosensors and HPLC analysis,
(ii) quantifying NO3- consumption and N2O production in gut and sediment using the 15N isotope pairing technique and gaschromatography, and
(iii) determining community structure and gene expression of denitrifying bacteria in gut and sediment using the genes coding for NO3- reductase (narG) and N2O reductase (nosZ) as well as their mRNA transcripts.
We will test the "mobile microsite" hypothesis in laboratory experiments, using freshwater sediment stocked with C. ripariuslarvae. The general applicability of the hypothesis will be evaluated at several NO3- polluted field sites in Denmark using sediment enclosures experimentally enriched in C. riparius larvae. These investigations will be complemented b y screening other benthic detritivores present at the field sites for denitrification and N2O emission. We expect that the results of this study will improve predictive models of eutrophication (e.g. NO3- dynamics) and global climate change (e.g. N2O emission).
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences ecology
- natural sciences earth and related environmental sciences atmospheric sciences climatology climatic changes
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
AARHUS C
Denmark
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