Objective
The main aim of the project is the further development of the applicant's skills and expertise in the application of the surface plasmon resonance imaging technique (SPR) for the investigation of interactions of proteins and other bioactive compounds.
The project consists of two particular targets:
i) the introduction of the SPR imaging technique to the host laboratory (University in Bialystok, Poland) in order to enhance host research potential in the area of interactions between cholesterol, phospholipids and cellular membrane proteins,
ii) development of a sensor for different cathepsins. Cholesterol is the major component of cell membranes. It also plays a crucial role in arteriosclerosis development.
The construction of a sensor with cholesterol immobilized on the sensor surface has potential for the investigation of cholesterol- protein interactions. Cholesterol mono-succilic acid will be immobilized to the thiol first layer on the gold chip. The immobilization will be performed with EDC and NHS application. Cathepsins are enzymes - lysosomal cysteine proteases, which play an important role in protein degradation, antigen presentation, bone resorption and hormone processing. These enzymes have also been implicated in tumour invasion and methastasis. Developing a sensor for cathepsins is considered ideal for the application of the SPR imaging system.
In order to develop a sensor for different cathepsins, the strong interaction of cathepsins with cystatin will be used. Cystatin C seems to be the most suitable for this purpose. In order to immobilize cystaine C, different thiols will be investigated as the first layer on gold. The active group of immobilized thiol is the carboxy group or the amine group. These groups react with protein and create covalent bonds. The linear Surface Plasmon Resonance system will be used for measurements consisting of a He-Ne laser, two polarizators, and two lenses for obtaining polarization light.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences condensed matter physics quasiparticles
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences chemical sciences organic chemistry amines
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- natural sciences physical sciences optics laser physics
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-11
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
BIALYSTOK
Poland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.