Objective
Plus-end tracking proteins, or +TIPs, are microtubule (MT) binding proteins that specifically associate with the distal ends of MTs. +TIPs are both involved in the regulation of MT dynamics and intracellular membrane transport over MTs. Since +TIPs are import ant mediators of MT dynamics during axonal growth, we speculate that they might also have a role in the establishment of synaptic contacts. This will be addressed by using +TIP knockout mice as model systems for abnormal synaptic formation and/or stabilization. Our studies will be performed in primary hippocampal neuronal cultures from these mice and will be supported by siRNA experiments.
The finding of abnormal synaptic contacts in +TIP deficient cells might be due to a deregulation of MT dynamics and/or to altered transport of synaptic proteins. Both questions will be addressed by using immuno-fluorescence time-lapse microscopy and by the use of a new microscope that allows the analysis of vesicle movement in living cells. Finally, we will study how distinct signal ling pathways involving the kinases GSK-3 and Abl lead to +TIP-mediated local rearrangements of MTs.
Biochemical studies will be performed in neuroblastoma and non-neuronal cell lines. The role of these signalling pathways during synaptogenesis will be evaluated in cultures of mossy fibres stimulated with Wnt7a. We expect that the results obtained from this project will help to improve our understanding of +TIP function during the highly complex process of synapse formation.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences neurobiology
- natural sciences chemical sciences inorganic chemistry alkali metals
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-11
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
ROTTERDAM
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.