Objective
Biological nitrogen fixation represents an economical and environmentally-friendly alternative to chemical fertilization. Anaspect of the rhizobia-legume symbiosis with important implications in other fields of plant biology is the molecular basis thatdiff erentiate symbiosis and pathogenesis. In plants under pathogen attack, there is a rapid and transient increase in theproduction of reactive oxygen species ("oxidative burst"). In contrast, during the symbiotic interaction, rhizobia may escape orinhibit the plant's defense response possibly due to bacterial Nod factors and, possibly, polysaccharides. However, hydrogenperoxide has been detected in the infection threads and nodule apoplast, suggesting that it is involved in several steps ofnodulation, such as the growth of cell walls and infection threads. Our previous studies have also shown that nodules containhigh levels of all three superoxide dismutases (CuZnSOD, FeSOD, MnSOD) and, based on co-localization and inhibitorstudies, we have proposed that CuZnSO D may be a source of hydrogen peroxide in nodules. The general objective of thisthree-year project is to study in detail the functions of SODs in nodule development. Using Medicago sativa and Lotuscorniculatus to compare indeterminate and determinate nodul ation, we will determine: (1) the regulatory mechanisms ofexpression for the three sod genes in nodules; (2) the spatio-temporal patterns of activity for the sod promoters; and (3) theeffect of suppression of SOD expression in nodules. To accomplish these specific objectives, a multidisciplinary and innovativeapproach and state-of-the-art techniques will be used. Methodologies include gene-specific quantification of transcripts forSODs and other antioxidant enzymes by real time RT-PCR, cytochemical localiza tion of superoxide and hydrogen peroxide,fusion constructs between promoters and reporter genes, transgenic roots and nodules ("hairy root" system), and genesil'
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules carbohydrates
- natural sciences biological sciences biological behavioural sciences ethology biological interactions
- natural sciences biological sciences genetics chromosomes
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds legumes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-11
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
MADRID
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.