Objective
We will use systems biology approaches to model RNA metabolism in yeast to aid understanding of these complex cellular pathways. In order to develop kinetic models, we propose to quantify RNA precursors and directly determine their rates of production as w ell as their processing and degradation through the various post-transcriptional pathways. Starting with ab-initio models describing the processing and degradation of yeast pre-messenger RNAs and pre-ribosomal RNAs, we will produce two comparable mathemati cal representations and populate the parameters using quantitative experimental data. Manipulation of the parameters will permit predictions to be made about the behaviour of the systems. These will be tested experimentally, using yeast mutants that block specific steps, or chemical inhibitors identified in high-throughput screens. Imaging techniques will be refined to visualise individual transcripts to determine whether the population data reflect the situation in individual cells. Comparison of the perfo rmance of the two models should provide further insights and enrich our understanding of both pathways. In addition, modelling precursor RNA processing in yeast will be of great benefit for understanding of these pathways in human cells, which are less ame nable to direct experimentation, and their significance for human genetic disorders.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-LIFESCIHEALTH-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
EDINBURGH
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.