Objective During the last five years, within the EU FP6 Integrated Project XENOME (LSHB-CT-2006-037377), we have convincingly demonstrated in primates that a complete control of induced type 1 diabetes is routinely obtained for 6/8 months after transplantation of subcutaneous alginate macroencapsulated porcine islets xenograft without the use of any immunosuppression. In order to improve the function of pig islets, we have produced with AVANTEA a transgenic pig expressing GLP-1 at the level of the pig islets. In fact, GLP1 is not only able to (i) increase the insulin gene expression and insulin biosynthesis, but also to (ii) induce the replication of islet cells and promotes islet-cell neogenesis and (iii) protect islets cells from apoptosis. GLP-1 has already demonstrated its in vivo potential to improve insulin secretion in subjects with impaired glucose tolerance and type 2 diabetes. New vectors (GLP1/M3Rr) containing both GLP1 sequence and muscarinic receptor mutation (M3R) will also be tested. The main objectives of this four-year project will therefore be (1) to evaluate in vitro the effect of GLP1/M3R transgene expression in pig islets after hyperglycaemic challenge; (2) to continue to produce double transgenic pigs specifically expressing GLP-1/M3R in pig islets under insulin promoter, and (3) finally to test these modified pig islets in our well characterized pig-to-diabetic primates model in vivo. However, in order to use islets from newborn unmodified or transgenic pigs, we will also set up (4) the pig islets isolation and cell maturation technique for Neonates Pancreatic Pig Cluster cells since neonates pig islets have prolonged survival and would also render possible the use of Designed Pathogen Free (DPF) neonates pigs which will represent a key point to reach clinical studies. BIOT will be mainly involved in this part of the work which is in vitro purification, amplification of NPCCS. GCU and AVIDIN Ltd will together develop arrays and a set of molecular testing for pig cell pathogens.A pilot study for safety will be achieved whether all the prerequisite are achieved at the end of the fourth year project. Fields of science natural sciencesbiological scienceszoologymammalogyprimatologymedical and health sciencesclinical medicineendocrinologydiabetesnatural sciencesbiological sciencesgeneticsmutationmedical and health sciencesclinical medicinetransplantation Programme(s) FP7-HEALTH - Specific Programme "Cooperation": Health Topic(s) HEALTH.2013.0-1 - Boosting the translation of health research projects' results into innovative applications for health Call for proposal FP7-HEALTH-2013-INNOVATION-2 See other projects for this call Funding Scheme CP-FP - Small or medium-scale focused research project Coordinator UNIVERSITE CATHOLIQUE DE LOUVAIN Address Place de l universite 1 1348 Louvain la neuve Belgium See on map Region Région wallonne Prov. Brabant Wallon Arr. Nivelles Activity type Higher or Secondary Education Establishments Administrative Contact Pierre Gianello (Prof.) Links Contact the organisation Opens in new window Website Opens in new window EU contribution € 1 641 678,89 Participants (4) Sort alphabetically Sort by EU Contribution Expand all Collapse all THE GLASGOW CALEDONIAN UNIVERSITY United Kingdom EU contribution € 731 660,00 Address Cowcaddens road, city campus 70 G4 0BA Glasgow See on map Region Scotland West Central Scotland Glasgow City Activity type Higher or Secondary Education Establishments Administrative Contact Linda Scobie (Dr.) Links Contact the organisation Opens in new window Website Opens in new window AVANTEA srl Italy EU contribution € 914 500,00 Address Via cabrini 12 26100 Cremona See on map Region Nord-Ovest Lombardia Cremona Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Giovanna Lazzari (Dr.) Links Contact the organisation Opens in new window Website Opens in new window BIOTALENTUM TUDASFEJLESZTO KFT Hungary EU contribution € 971 000,00 Address Aulich lajos utca 26 2100 Goedoello See on map Region Közép-Magyarország Pest Pest Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Andras Dinnyes (Prof.) Links Contact the organisation Opens in new window Website Opens in new window AVIDIN KUTATO, FEJLESZTO ES KERESKEDELMI KFT Hungary EU contribution € 778 309,11 Address Also kikoto sor 11 6726 Szeged See on map Region Alföld és Észak Dél-Alföld Csongrád Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Laszlo Puskas (Dr.) Links Contact the organisation Opens in new window Website Opens in new window
