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Content archived on 2024-05-28

Assembly and coordination of peptidoglycan synthesis complexes during bacterial growth and cell division

Objective

In this project, I will investigate the proteins involved, and their interactions, in the formation of both peptidoglycan (PG) synthesis core complexes (elongasome and septosome) in the model bacterium Escherichia coli to gain new molecular insights into how the cell grows its cell wall during the cell cycle. This will be achieved by: (i) in vivo characterization of new or already known important interactions within or between the PG synthesis machineries. This part of the project will provide relevant information about the hierarchy or requirement between the proteins involved in the process, which is essential information for the following more detailed mechanistic studies; (ii) studying the PG protein interactions and enzymatic activities during the intermediate step between the elongation and the cell division processes, which will provide new insight in how elongasome and septosome complexes are connected and regulated during the cell cycle, one of the most important unknown processes in the research area; and (iii) reconstructing the elongasome and septosome activities in a more natural environment to quantify the activities and study the properties of each protein involved. During the proposed project, I will obtain knowledge and skills in purification and handling of membrane proteins, in vitro reconstitution approaches and cutting-edge techniques available in the host lab and institute. The top-ranked host lab and collaborators will provide me the best environment for my scientific development, enhancing my competences in the field.

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-2013-IEF
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

UNIVERSITY OF NEWCASTLE UPON TYNE
EU contribution
€ 221 606,40
Address
KINGS GATE
NE1 7RU Newcastle Upon Tyne
United Kingdom

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Region
North East (England) Northumberland and Tyne and Wear Tyneside
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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