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Mechanical loading to direct stem cell differentiation

Objective

The main goal of this proposal is to induce and detect in real time and at single-cell level the differentiation of human mesenchymal stem cells using mechanical loads in 2D and 3D conditions. This proposal integrates disciplines from nanotechnology, bioengineering and cell and molecular biology. First we propose to develop a method to track the differentiation of stem cells in real time and at the single cell level using cytoskeletal organization of actin, microtubules and intermediate filaments as a suitable cell biomarker. We will then establish mechanical loading protocols to induce, via direct force application onto cells, the first stages of stem cell differentiation towards specific cell lineages. We will apply cyclic tensile strain and compression to stem cells in 2D and 3D conditions, and track their differentiation status in real time using the cytoskeletal biomarkers that we will have identified before. The results of this proposal will have implications for the field of stem cell mechanobiology in particular, and some of the techniques developed will also contribute to the wider field of directed stem cell differentiation.

Field of science

  • /medical and health sciences/medical biotechnology/cells technologies/stem cells
  • /engineering and technology/nanotechnology
  • /natural sciences/biological sciences/molecular biology

Call for proposal

FP7-PEOPLE-2013-CIG
See other projects for this call

Funding Scheme

MC-CIG - Support for training and career development of researcher (CIG)

Coordinator

QUEEN MARY UNIVERSITY OF LONDON
Address
327 Mile End Road
E1 4NS London
United Kingdom
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 100 000
Administrative Contact
Greg Dow (Mr.)