Objective The main goal of this proposal is to induce and detect in real time and at single-cell level the differentiation of human mesenchymal stem cells using mechanical loads in 2D and 3D conditions. This proposal integrates disciplines from nanotechnology, bioengineering and cell and molecular biology. First we propose to develop a method to track the differentiation of stem cells in real time and at the single cell level using cytoskeletal organization of actin, microtubules and intermediate filaments as a suitable cell biomarker. We will then establish mechanical loading protocols to induce, via direct force application onto cells, the first stages of stem cell differentiation towards specific cell lineages. We will apply cyclic tensile strain and compression to stem cells in 2D and 3D conditions, and track their differentiation status in real time using the cytoskeletal biomarkers that we will have identified before. The results of this proposal will have implications for the field of stem cell mechanobiology in particular, and some of the techniques developed will also contribute to the wider field of directed stem cell differentiation. Fields of science engineering and technologynanotechnologymedical and health sciencesmedical biotechnologycells technologiesstem cellsnatural sciencesbiological sciencesmolecular biology Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) FP7-PEOPLE-2013-CIG - Marie-Curie Action: "Career Integration Grants" Call for proposal FP7-PEOPLE-2013-CIG See other projects for this call Funding Scheme MC-CIG - Support for training and career development of researcher (CIG) Coordinator QUEEN MARY UNIVERSITY OF LONDON EU contribution € 100 000,00 Address 327 MILE END ROAD E1 4NS London United Kingdom See on map Region London Inner London — East Tower Hamlets Activity type Higher or Secondary Education Establishments Administrative Contact Greg Dow (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
