Periodic Reporting for period 3 - TEC_Pro (Molecular control of self-renewal and lineage specification in thymic epithelial cell progenitors in vivo.)
Reporting period: 2018-07-01 to 2019-12-31
My objectives are to identify TEC progenitors and their niches within the thymus, define new molecular components involved in their self-renewal and lineage potential, and elucidate the genetic programs that regulate cTEC/mTEC fate decision. We take a global approach to examine TEC differentiation, which integrates the study of molecular processes taking place at cellular level and the analysis of in vivo mouse models. Using advanced research tools that combine reporter mice, clonogenic assays, organotypic cultures, high-throughput screen and genome-wide transcriptomic profiling, we are dissecting the principles that underlie the self-renewal and lineage differentiation of TEC progenitors in vivo.