Periodic Reporting for period 4 - SegregActin (Building Distinct Actin Filament Networks in a Common Cytoplasm)
Reporting period: 2018-11-01 to 2020-04-30
The objective of this project is to understand 1/ how cells can generate the formation of actin structures of appropriate ABP composition from a common pool of cytoplasmic components and 2/ the relationship between the ABP composition of an actin network, its geometrical and dynamical properties, and its response to mechanical deformations.
• Development of super-resolution TIRF imaging to evaluate the range of allosteric interactions along an actin filament.
• Framework for stochastic in silico simulations to understand how families of actin binding proteins segregate on different actin networks.
• Reconstitution of branched Arp2/3 and linear formin actin networks in a common environment (bottom-up approach).
• Generation of a large collection of yeast mutants to later understand the effects, locally and globally, of the depletion of an ABP for a biological function (top-down approach).
• Adaptation of the use of yeast extract for branched network assembly to a force measurement device.
We successfully started almost all the parts of the projects and obtained interesting results. We expect that the first manuscripts from the team will be written and submitted soon.