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Plasmonic-based autOmated lab-on-chip SEnsor for the rapid In-situ Detection of LegiONella

Objective

Legionnaires’ disease is a serious form of pneumonia caused by bacterium Legionella pneumophila, with a case-fatality ratio on the order of 10-15%. L. pneumophila proliferates in aquatic habitats, especially in potable water, air conditioning, hot and cold water systems, cooling towers, evaporative condensers, spa/natural pools. Actually, its detection and monitoring rely on time-consuming protocols (in the order of several days) based on in-vitro selective bacteria culture methods, performed by highly specialized personnel in dedicated laboratories. POSEIDON project targets to change the approach in bacteriological environmental monitoring and in infection risk management by developing a fully automatic and reliable system. Handling of the air/water sample will be designed and integrated in preconditioning system and microfluidic device through which whole bacteria cells will be transported from the sampling module to the sensing plasmonic surface. The complete measure protocol will be integrated and performed according to EU legislation guidelines. Specificity will be ensured by immuno-functionalization of gratings surfaces and enhanced system sensitivity will be granted by the optimization of the optical detection system architecture. Sensors based on Grating Coupled Surface Plasmon Resonance (GC-SPR) in azimuthally rotating configuration have recently proved sensitivity enhancement up to almost two orders of magnitude. Furthermore, the symmetry breaking related to grating rotation allows exploiting the incident polarization, more easily controlled with respect to incidence wavelength and angles interrogation. The prototype will be designed to be integrated in water distribution or HVAC systems in order to demonstrate its feasibility in industrially relevant fields and to open new applications and new market opportunities. POSEIDON project aims to address new solutions in this relevant health and safety societal challenge.

Field of science

  • /social sciences/economics and business/business and management/commerce
  • /social sciences/sociology/governance/crisis management
  • /engineering and technology/mechanical engineering/thermodynamic engineering

Call for proposal

H2020-ICT-2014-1
See other projects for this call

Funding Scheme

RIA - Research and Innovation action

Coordinator

CLIVET SPA
Address
Via Camp Lonc 25 Frazione Villapai Era
32032 Feltre
Italy
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
EU contribution
€ 626 125

Participants (6)

VENETO NANOTECH SOCIETA CONSORTILEPER AZIONI

Participation ended

Italy
EU contribution
€ 92 252,68
Address
Piazza Salvemini 13
35131 Padova
Activity type
Research Organisations
CATLAB-CENTRE ANALITIQUES TERRASSA AIE
Spain
EU contribution
€ 317 500
Address
Vial Sant Jordi S/n
08232 Viladecavalls
Activity type
Other
UPPSALA UNIVERSITET
Sweden
EU contribution
€ 787 031
Address
Von Kraemers Alle 4
751 05 Uppsala
Activity type
Higher or Secondary Education Establishments
METROHM-APPLIKON
Netherlands
EU contribution
€ 1 139 500
Address
De Brauwweg 13
3125AE Schiedam
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
ARC - CENTRO RICERCHE APPLICATE
Italy
EU contribution
€ 733 812,51
Address
Viale Della Navigazione Interna 51
35129 Padova
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
PROTOLAB SRL
Italy
EU contribution
€ 372 559,81
Address
Via Rampazzo 3
35129 Padova
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)