Within Stefy-Phase-1 funded by H2020SMEINST-1-2014, Proxentia srl assessed the technical and economic feasibility on the innovation proposed. The operational capacity of all the steps necessary for device components production and the further scale up has been demonstrated, ensuring the constant repeatability of the technology performance. About 400 SiO2-coated glasses, which are at the basis of the Stefy technology (Reflective Phantom Interface), have been prepared and tested for their optical activity. After demonstrating the scalability of the coating process, about 400 sensing surfaces have been prepared binding specific antibodies to detect selected molecules target. Stefy demonstration, in fact, focused on the three main applications foreseen for Stefy exploitation and commercialization: the detection of a) toxins in flour; b) toxins and allergens in wine and c) allergens in processed foodstuff . The sensing surfaces prepared have been embedded into disposable cuvettes and sensibility detection tests against different molecules have been performed. In real wine samples, tests confirmed the outstanding limit of detection of 0.5 g/ml for allergens (being from 1 to 10 g/ml the limit required by the legislation). Even higher sensibility of detection, 50 pg/ml, has been demonstrated for Alfatoxin, (being from 2 to 10 ng/ml, admitted by the legislation).