Automated synthesis of S. pneumoniae 7F capsular polysaccharide repeating unit as candidate for conjugate vaccines

Objective

The goal of this project is to develop an automated synthesis of the Streptococcus pneumoniae 7F capsular polysaccharide repeating unit, which is of high interest for synthetic vaccines against this pathogen. This structural motif has never been synthesized yet, not even by traditional solution techniques. Our approach is highly innovative in the application of automated oligosaccharide synthesis, which will make the synthetic procedure more time- and cost-effective.
In the course of the project, a series of orthogonally protected monosaccharide building blocks will be synthesized. The oligosaccharide will be assembled on oligosaccharide synthesizer. Photocleavage in a flow reactor and global deprotection will yield the target molecule. The oligosaccharide will be subjected to bioconjugation with a CRM197 carrier protein to produce conjugate vaccine candidate. This conjugate will be tested for the ability to induce an immune response against S. penumoniae 7F.

Fields of science

• medical and health sciences › basic medicine › immunology
• natural sciences › biological sciences › molecular biology
• medical and health sciences › basic medicine › pharmacology and pharmacy › pharmaceutical drugs › vaccines
• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• natural sciences › biological sciences › biochemistry › biomolecules › carbohydrates

Programme(s)

• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility

Topic(s)

• MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF)

Call for proposal

H2020-MSCA-IF-2014
See other projects for this call

Funding Scheme

MSCA-IF-EF-ST - Standard EF

Coordinator

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV

Address

Hofgartenstrasse 8
80539 Munchen
Germany

Activity type

Research Organisations

EU contribution

€ 159 460,80