Objective Plants are rich sources of nutrients and water for diverse microbial communities. Some of these communities evolved parasitism as a strategy to access plant nutrients, with devastating results for crops. Plants are protected from infection by a waxy cuticular layer above the walls of epidermal cells. Would-be pathogens breaching this barrier, or entering via stomata, encounter an active plant immune system that specifically recognizes pathogens. Breaching leads to the deployment of two synergistic pathways that orchestrate immune responses. The first relies on the detection of pathogen-associated molecular patterns (PAMPs) and culminates in pattern-triggered immunity (PTI). When the first is circumvented a second array of responses takes place known as effector triggered immunity (ETI). In ETI, host factors known as R proteins recognize pathogen effectors, an event which is accompanied by the execution of a unique programmed cell death (PCD) type known as the hypersensitive response (HR). Although the initiator of the HR-PCD is known to depend on the formation of an effector-R complex, the downstream molecular events remain elusive. Previous results showed that particular proteases known as metacaspases (MCs) modulate HR-PCD, highlighting the importance of proteolysis and proteome rearrangements for HR-PCD modulation. I will attempt to shed light on the rearrangements of the HR-PCD proteome landscape, by studying processes that control it: selective RNA decapping and translation and proteolytic events, in a highly temporal manner using systematic approaches and reverse genetics. This project is expected to elucidate the importance of these processes and provide a detailed analysis of mRNA and protein level rearrangements during HR-PCD. In addition, this project will suggest strategies for enhancement of plant immunity against pathogens, which is urgently needed to sustain food security considering the ever growing earth’s population. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsmedical and health scienceshealth sciencesnutritionnatural sciencesbiological sciencesbiological behavioural sciencesethologybiological interactionsnatural sciencesbiological sciencesgeneticsRNAnatural scienceschemical sciencesanalytical chemistrymass spectrometry Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF) Call for proposal H2020-MSCA-IF-2014 See other projects for this call Funding Scheme MSCA-IF-EF-ST - Standard EF Coordinator THE SAINSBURY LABORATORY Net EU contribution € 183 454,80 Address Norwich research park, colney lane NR47UH Norwich GB See on map Region East of England East Anglia Norwich and East Norfolk Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00
