Objective
The research programme will comprise the study of the Histone acetyl transferase (HAT) MOZ (Monocytic leukaemia Zinc finger) a critical player in the development and maintenance of the haematopoietic system. In addition, translocations of MOZ are associated with Acute Myeloblastic Leukaemia. Given the importance of this protein in haematopoiesis and leukaemogenesis, it would be essential to further understand the function of MOZ and in particular to identify the genes regulated by its HAT activity of MOZ. A mouse line specifically mutated to abrogate the HAT enzymatic activity of MOZ has been developed in the host laboratory, and represents a powerful and unique tool for the study of MOZ HAT activity. Using Chromatin ImmunoPrecipitation followed by sequencing (ChIP-seq), genome wide targets of MOZ will be determined and the consequences of the deletion of its HAT activity on the epigenetic landscape will be investigated through the survey of several histone modifications and RNA polymerase II recruitment. The non-histone targets of MOZ will be identified through the SILAC technique. Finally a MOZ murine leukaemic model has been developed, based on the transduction of bone marrow cells with a retrovirus encoding MOZ-TIF2, one of the fusion proteins involving MOZ. ChIP-seq will be performed in these leukaemic cells to identify genes targeted by MOZ-TIF2 and changes in epigenetic marks at these loci. Overall, this project will provide a better understanding of MOZ HAT activity in haematopoiesis and leukaemogenesis. Crucially, this project will enable Dr Anne Largeot to obtain and develop a wide range of new and transferable skills through a variety of multidisciplinary training programs. Relying on the knowledge and expertise of her supervisor and the exceptionally high calibre of the Cancer Research UK Manchester Institute, this project will enable Dr Anne Largeot to achieve her long-term objective of obtaining a leadership position in a competitive European Institute
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences chemical sciences inorganic chemistry transition metals
- natural sciences biological sciences developmental biology
- medical and health sciences medical biotechnology cells technologies stem cells
- medical and health sciences clinical medicine oncology leukemia
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2014
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
M13 9PL Manchester
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.