Objective
Next generation sequencing (NGS) has become an indispensable tool for system biology and has recently been adopted in protein engineering science as well. This proposal describes the development of a highly generalizable technique, termed FUNC-NGS, which links in situ functional information of protein activity with the function-determining DNA sequence in a massively parallel manner opening exciting possibilities, e.g. in drug discovery. In this method a cell-free display platform is combined with NGS by modifying protein display units to contain unique homing oligonucleotide sequences. These units are subsequently targeted to bind monoclonal DNA clusters in the flow cell of an Illumina sequencer by complementary DNA hybridization. FUNC-NGS is readily compatible with a paired-end sequencing programme on the Illumina platform and the number of screening units is only limited by the number of DNA clusters on the flow cell. As the first application challenge, a new variant of a modified bacterial adhesin, Spycatcher, will be created with an altered specificity for synthetic biology applications. Novel Spycatcher-SpyTag pairs are intensively desired but their creation is demanding. Solving this problem will be a perfect showcase for this radically novel deep screening technology. At the mature stage FUNC-NGS will be a transformative platform in therapeutic protein development, functional genomics and personalized medicine. The proposed work will be carried out at Dr. Hollfelder´s laboratory at the University of Cambridge with a short secondment at Medimmune. Hollfelder group has developed SNAP display, which is a FUNC-NGS-compatible in vitro protein display system. Furthermore, the University hosts excellent facilities for NGS sequencing and experienced bioinformatics support is available. The planned research programme synergistically combines my expertise in molecular biology method development with the hosts expertise in cell-free display and IVC-techniques.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences synthetic biology
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences molecular biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2014
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
CB2 1TN CAMBRIDGE
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.