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High-throughput characterization of host promiscuity for precisely designed synthetic viral capsid

Objective

Traditional geographic ranges of insect pests are altered by globalization and climate change, resulting in emerging threats for natural ecosystems, agriculture and human health. It is urgent to improve our ability to swiftly develop targeted solutions to contain such threats, while minimizing unintended side effects on the environment.
Densovirinae are very small, single-stranded DNA viruses pathogenic to a variety of arthropods, including pythophagous caterpillars and grasshoppers, as well as disease vectors such as aphids and mosquitoes. Host ranges appear to vary considerably amongst even closely related densoviruses. A better understanding of the molecular mechanisms underlying virulence and specificity is necessary to evaluate the risk and opportunities associated with potential use in controlling natural insect populations.

We propose to discover these molecular determinants through the use of precisely designed libraries of viral genomes. We will leverage cost-effective and scalable DNA synthesis and sequencing technologies to generate high-throughput implementation and testing of precise molecular hypotheses. These will be informed by currently available knowledge and further deepened by bioinformatic analysis of natural sequences. We will produce ~106 controlled variants of the viral capsid as well as select viral regulatory sequence elements (enhancers, splicing and translation start sites). Resulting libraries will be subjected to multiplexed, deep-sequencing-based functional assays.

The scale of this approach will permit to reconstitute the sequence-structure-activity relationships required to relate viral genomes to host specificity and propagation efficiency. These data will support the development of models to predict the behavior of a viral genome in a new ecology, assess its potential for future evolution and inform the safe use of viral vectors for the targeted biocontrol of insect populations.

Field of science

  • /natural sciences/biological sciences/genetics and heredity/genome
  • /natural sciences/biological sciences/zoology/entomology
  • /social sciences/sociology/globalization
  • /natural sciences/biological sciences/microbiology/virology
  • /medical and health sciences/health sciences/infectious disease/DNA virus
  • /natural sciences/biological sciences/synthetic biology
  • /natural sciences/biological sciences/ecology/ecosystems
  • /agricultural sciences/agriculture, forestry, and fisheries/agriculture
  • /natural sciences/biological sciences/genetics and heredity/mutation
  • /medical and health sciences/medical biotechnology/genetic engineering/gene therapy
  • /natural sciences/earth and related environmental sciences/atmospheric sciences/climatology/climatic changes
  • /natural sciences/biological sciences/zoology/invertebrate zoology

Call for proposal

H2020-MSCA-IF-2014
See other projects for this call

Funding Scheme

MSCA-IF-EF-ST - Standard EF

Coordinator

INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
Address
147 Rue De L'universite
75007 Paris Cedex 07
France
Activity type
Research Organisations
EU contribution
€ 185 076