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Single cell biophysics of bacterial cell shape

Objective

In bacteria, the rigid external cell wall (CW) and the intracellular actin-like (MreB) cytoskeleton are major determinants of cell shape. Synthesis and chemical composition of the CW, a three dimensional polymer network that is one of the most prominent targets for antibiotics, are well understood. However, despite decades of study, little is known about the complex CW ultrastructure and the molecular mechanisms that control cell shape in time and space. MreB homologues assemble into dynamic membrane-associated structures thought to control shape by serving as organizers for the movement and assembly of macromolecular machineries responsible for CW biogenesis. However, the mechanistic details used by the MreB cytoskeleton to fulfill this role remain to be elucidated.
We will combine powerful genetic tools available in the model Gram-positive bacterium Bacillus subtilis with modern high-resolution fluorescence microscopy techniques and atomic force microscopy (AFM) to study the role of the MreB cytoskeleton and CW synthesis proteins in cell shape determination and maintenance. Additionally, the role of mechanical forces in the control of CW organization will be evaluated.

Field of science

  • /medical and health sciences/basic medicine/physiology/homeostasis
  • /natural sciences/chemical sciences/polymer science
  • /natural sciences/physical sciences/optics/microscopy
  • /natural sciences/biological sciences/microbiology/bacteriology
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes
  • /natural sciences/chemical sciences/inorganic chemistry/inorganic compounds

Call for proposal

H2020-MSCA-IF-2014
See other projects for this call

Funding Scheme

MSCA-IF-EF-RI - RI – Reintegration panel

Coordinator

INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
Address
147 Rue De L'universite
75007 Paris Cedex 07
France
Activity type
Research Organisations
EU contribution
€ 185 076