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Single cell biophysics of bacterial cell shape

Objective

In bacteria, the rigid external cell wall (CW) and the intracellular actin-like (MreB) cytoskeleton are major determinants of cell shape. Synthesis and chemical composition of the CW, a three dimensional polymer network that is one of the most prominent targets for antibiotics, are well understood. However, despite decades of study, little is known about the complex CW ultrastructure and the molecular mechanisms that control cell shape in time and space. MreB homologues assemble into dynamic membrane-associated structures thought to control shape by serving as organizers for the movement and assembly of macromolecular machineries responsible for CW biogenesis. However, the mechanistic details used by the MreB cytoskeleton to fulfill this role remain to be elucidated.
We will combine powerful genetic tools available in the model Gram-positive bacterium Bacillus subtilis with modern high-resolution fluorescence microscopy techniques and atomic force microscopy (AFM) to study the role of the MreB cytoskeleton and CW synthesis proteins in cell shape determination and maintenance. Additionally, the role of mechanical forces in the control of CW organization will be evaluated.

Fields of science (EuroSciVoc)

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Topic(s)

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Funding Scheme

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MSCA-IF-EF-RI - RI – Reintegration panel

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2014

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Coordinator

INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 185 076,00
Address
147 RUE DE L'UNIVERSITE
75007 PARIS CEDEX 07
France

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Region
Ile-de-France Ile-de-France Paris
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 185 076,00
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