Objective
Cell membrane permeabilization, involving its reversible or irreversible restructuring, under extremely short electric (E) pulses exposure (from hundreds of us down to few ns) has a key role in a number of clinical, preclinical, and medical research applications. Hence, the knowledge of its bio-physical nature and dynamics (i.e. formation and resealing processes) is crucial for an aware control of delivered protocols, and for predicting treatments efficacy under established biological (e.g. buffer conductivity, cell dimensions) and electromagnetic (EM) conditions (e.g. pulse duration, amplitude, shape). To understand bio-physical mechanisms underlying such a pulse-exposure permeabilization, an innovative and advantageous approach is proposed based on real-time molecular vibrational modes imaging using fast Coherent anti-Stokes Raman Scattering (CARS) of in vitro samples under such a stimulation. Hence, in the present Marie Sklodowska Curie Action (MSCA), a fast CARS microscope will be integrated with a suitable wide band in vitro electromagnetic field (EMF) exposure setup to study the role of membrane lipid reactions and interfacial water in the electropermeabilization phenomenon. This innovative CARS analysis will be supported by mass spectroscopy measurements to verify the appearance of corresponding chemical species, and by simulated E field and pore density distributions on the bio-samples. This last step is an attempt to correlate sample areas presenting the highest CARS signal intensity with the correspondent E field and pore density distributions. The program that integrates engineering, physics, chemistry and biology will allow a substantial progression in my career and network ability through the work on a completely innovative, multidisciplinary and inter-disciplinary subject in one of the most reputed bioelectric laboratory, where the applications of electropermeabilization in biology and medicine where first developed in Europe and worldwide.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences electromagnetism and electronics electromagnetism
- natural sciences chemical sciences electrochemistry electrolysis
- natural sciences physical sciences optics microscopy
- natural sciences biological sciences biochemistry biomolecules lipids
- natural sciences chemical sciences electrochemistry bioelectrochemistry electroporation
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2014
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
75794 PARIS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.