Objective The project aims to create a demo system for cost effective, non-invasive device for rapid detection of cystic fibrosis inhumans.The detection of human recessive diseases has been dominated by the use of fluorescent biomarkers, based on organicdyes, helping researchers to study and analyse gene expression, cell cycle, and enzymatic activity. Among severalproteolytic enzymes, trypsin has attracted much attention, as it is a target in the study of various important human recessivediseases including, for example, cystic fibrosis (CF).We present herein two colour encoded silica nanospheres (2nanoSi) for the fluorescence quantitative ratiometricdetermination of cystic in humans. Current detection technologies for cystic fibrosis diagnosis are slow, costly and sufferfrom false positives. The 2nanoSi proved to be a fast (minutes), a single-step and with two times higher sensitivity than thestate-of-the-art biomarkers based sensors for cystic fibrosis, allowing the quantification of trypsin concentrations in a widerange (25-350 μg/L). Moreover, our approach can be used from the 4th day of life when the trypsin concentration is alreadythe same as in adults. Furthermore, as trypsin is directly related to the development of cystic fibrosis (CF), different humanphenotypes, i.e. normal (160-340 μg/L), CF homozygotic (0-90 μg/L), and CF heterozygotic (91-349 μg/L), respectively, canbe determined using our 2nanoSi nanospheres. We anticipate the 2nanoSi system to be a starting point for non-invasive,easy-to-use and cost effective ratiometric fluorescence biomarker for recessive genetic diseases alike human cystic fibrosis. Fields of science engineering and technologymaterials engineeringcolorsengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programme(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Topic(s) ERC-PoC-2014 - ERC Proof of Concept Grant Call for proposal ERC-2014-PoC See other projects for this call Funding Scheme ERC-POC - Proof of Concept Grant Coordinator FUNDACIO PRIVADA INSTITUT CATALA D'INVESTIGACIO QUIMICA Net EU contribution € 101 000,00 Address Avenida paissos catalans 16 43007 Tarragona Spain See on map Region Este Cataluña Tarragona Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 Beneficiaries (3) Sort alphabetically Sort by Net EU contribution Expand all Collapse all FUNDACIO PRIVADA INSTITUT CATALA D'INVESTIGACIO QUIMICA Spain Net EU contribution € 101 000,00 Address Avenida paissos catalans 16 43007 Tarragona See on map Region Este Cataluña Tarragona Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 CORPORACIO SANITARIA PARC TAULI DE SABADELL Spain Net EU contribution € 36 468,75 Address Parc tauli s/n 08208 Sabadell See on map Region Este Cataluña Barcelona Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00 Third-party Legal entity other than a subcontractor which is affiliated or legally linked to a participant. The entity carries out work under the conditions laid down in the Grant Agreement, supplies goods or provides services for the action, but did not sign the Grant Agreement. A third party abides by the rules applicable to its related participant under the Grant Agreement with regard to eligibility of costs and control of expenditure. FUNDACIO INSTITUT D'INVESTIGACIÓ I INNOVACIÓ PARC TAULI Spain Net EU contribution € 12 531,25 Address Parc tauli 1 08208 Sabadell See on map Region Este Cataluña Barcelona Activity type Other Links Contact the organisation Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00
