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New Diagnostics for Infectious Diseases: “ND4ID”


E-Bug Educational packages

E-Bug as a means to reaching out to young students: The e-Bug project ( is a DG SANCO funded project established in 2006 and involves a consortium of 28 countries, in Belgium led by partner 1 (UA). The project aims to educate children and young people, at junior and senior school level, about microbiology, hygiene and the spread, treatment and prevention of infectious diseases. Our ESRs will make use of this platform to develop 3 educational packages (lesson packs, fact sheets, quiz, games, home science project) related to IVDs. As the E-bug website and project are already well known throughout Europe and beyond, this platform will ensure optimal outreach and visibility of the project at our target group of young (future) scientists. Simultaneously, this will teach the ESRs how to translate their research into an easy-to-understand format and provides them with multimedia training.

ND4ID website, Twitter account and Facebook account with content

ND4ID website and social media presence of ND4ID and our ESRs: A public website, linked to a Linked-In, Facebook and Twitter account will be developed and maintained and will serve as our “one to many” communication tool informing all target groups about the project, its goals and activities. It will have links to related projects such as RAPP-ID, PREPARE, COMPARE, ROUTINE, COMBACTE, ND4BB and will serve as the repository for teaching and training material used during the training events, electronic versions of publications (open access), proceedings from the International Conference, PhD manuscripts and other (non-confidential) project related information. The website and social media will contain elements such as ESR blogs and vlogs, giving the audience a first hand account of their experiences. The website will also have a protected section containing working spaces and repositories for the network participants (e.g. for sharing of contact details, meeting minutes, organisation of training events, analysis protocols, preliminary reports, publications in progress.)

Training event 4

TECH3: Key Enabling Technologies TRANS2: Managing Innovation and Knowledge

Training event 3

CLIN2: Role of NGS in the clinical environment


CHALL1 Research plan The four ESR teams are given the assignment to draft a clear research plan on how to validate (analytical, clinical) the results of their WP and report on ethical and regulatory aspects, applying their knowledge of TRANS1. This will help them understand the validation and regulatory trajectory and associated time frame of diagnostic test development.

Training event 1

CLIN1: Clinical management of infectious diseases TECH1: BioAssay Development

Training event 2

TECH2: Sample Preparation TRANS1: Addressing Ethical, Regulatory and Legal aspects

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Capillary Pumping Independent of Liquid Sample Viscosity

Author(s): Weijin Guo†, Jonas Hansson†, and Wouter van der Wijngaart*
Published in: Langmuir, Issue 2016, 32 (48), 2016, Page(s) pp 12650–12655, ISSN 0743-7463

Bioelectrochromic hydrogel for fast antibiotic-susceptibility testing

Author(s): Ferran Pujol-Vila, Jiri Dietvorst, Laura Gall-Mas, María Díaz-González, Núria Vigués, Jordi Mas, Xavier Muñoz-Berbel
Published in: Journal of Colloid and Interface Science, Issue 511, 2018, Page(s) 251-258, ISSN 0021-9797
DOI: 10.1016/j.jcis.2017.09.004

Digital Rolling Circle Amplification–Based Detection of Ebola and Other Tropical Viruses

Author(s): Sibel Ciftci, Felix Neumann, Samir Abdurahman, K. Sofia Appelberg, Ali Mirazimi, Mats Nilsson, Narayanan Madaboosi
Published in: The Journal of Molecular Diagnostics, Issue 22/2, 2020, Page(s) 272-283, ISSN 1525-1578
DOI: 10.1016/j.jmoldx.2019.10.014

Padlock Probe Assay for Detection and Subtyping of Seasonal Influenza

Author(s): Felix Neumann, Iván Hernández-Neuta, Malin Grabbe, Narayanan Madaboosi, Jan Albert, Mats Nilsson
Published in: Clinical Chemistry, Issue 64/12, 2018, Page(s) 1704-1712, ISSN 0009-9147
DOI: 10.1373/clinchem.2018.292979

Silica bead-based microfluidic device with integrated photodiodes for the rapid capture and detection of rolling circle amplification products in the femtomolar range

Author(s): Ruben R.G. Soares, Felix Neumann, Catarina R.F. Caneira, Narayanan Madaboosi, Sibel Ciftci, Iván Hernández-Neuta, Inês F. Pinto, Denis R. Santos, Virginia Chu, Aman Russom, João P. Conde, Mats Nilsson
Published in: Biosensors and Bioelectronics, Issue 128, 2019, Page(s) 68-75, ISSN 0956-5663
DOI: 10.1016/j.bios.2018.12.004

Anti‐ADAMTS13 autoantibodies in immune‐mediated thrombotic thrombocytopenic purpura do not hamper ELISA‐based quantification of ADAMTS13 antigen

Author(s): Charlotte Dekimpe, Elien Roose, Claudia Tersteeg, Bérangère S. Joly, Aurélie Dewaele, Sara Horta, Inge Pareyn, Aline Vandenbulcke, Hans Deckmyn, Hendrik B. Feys, Edwige Tellier, Gilles Kaplanski, Marie Scully, Paul Coppo, Simon F. De Meyer, Agnès Veyradier, Karen Vanhoorelbeke
Published in: Journal of Thrombosis and Haemostasis, 2020, ISSN 1538-7933
DOI: 10.1111/jth.14747

A novel mutation tolerant padlock probe design for multiplexed detection of hypervariable RNA viruses

Author(s): Sibel Ciftci, Felix Neumann, Iván Hernández-Neuta, Mikhayil Hakhverdyan, Ádám Bálint, David Herthnek, Narayanan Madaboosi, Mats Nilsson
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
DOI: 10.1038/s41598-019-39854-3

Epidemiology and characterisation of carbapenem-non-susceptible Pseudomonas aeruginosa in a large intensive care unit in Jakarta, Indonesia

Author(s): Yulia Rosa Saharman, Andreu Coello Pelegrin, Anis Karuniawati, Rudyanto Sedono, Dita Aditianingsih, Wil H.F. Goessens, Corné H.W. Klaassen, Alex van Belkum, Caroline Mirande, Henri A. Verbrugh, Juliëtte A. Severin
Published in: International Journal of Antimicrobial Agents, Issue 54/5, 2019, Page(s) 655-660, ISSN 0924-8579
DOI: 10.1016/j.ijantimicag.2019.08.003

mcr-9 , an Inducible Gene Encoding an Acquired Phosphoethanolamine Transferase in Escherichia coli , and Its Origin

Author(s): Nicolas Kieffer, Guilhem Royer, Jean-Winoc Decousser, Anne-Sophie Bourrel, Mattia Palmieri, Jose-Manuel Ortiz De La Rosa, Hervé Jacquier, Erick Denamur, Patrice Nordmann, Laurent Poirel
Published in: Antimicrobial Agents and Chemotherapy, Issue 63/9, 2019, ISSN 0066-4804
DOI: 10.1128/aac.00965-19

Genomic Evolution of Staphylococcus aureus During Artificial and Natural Colonization of the Human Nose

Author(s): Manisha Goyal, Fabien Javerliat, Mattia Palmieri, Caroline Mirande, Willem van Wamel, Mehri Tavakol, Nelianne J. Verkaik, Alex van Belkum
Published in: Frontiers in Microbiology, Issue 10, 2019, ISSN 1664-302X
DOI: 10.3389/fmicb.2019.01525

PFM-Like Enzymes Are a Novel Family of Subclass B2 Metallo-β-Lactamases from Pseudomonas synxantha Belonging to the Pseudomonas fluorescens Complex

Author(s): Laurent Poirel, Mattia Palmieri, Michael Brilhante, Amandine Masseron, Vincent Perreten, Patrice Nordmann
Published in: Antimicrobial Agents and Chemotherapy, Issue 64/2, 2020, ISSN 0066-4804
DOI: 10.1128/aac.01700-19

Differences and overlaps between Phd studies in diagnostic microbiology in industrial and academic settings

Author(s): Alex van Belkum, Andreu Coello Pelegrin, Rucha Datar, Manisha Goyal, Mattia Palmieri, Caroline Mirande, Valérie Chalansonnet, Sylvain Orenga
Published in: Medical Microbiology and Immunology, 2019, ISSN 0300-8584
DOI: 10.1007/s00430-019-00643-4

DNA-only, microwell-based bioassay for multiplex nucleic acid detection with single base-pair resolution using MNAzymes

Author(s): Saba Safdar, Karen Ven, Julie van Lent, Benjamin Pavie, Iene Rutten, Annelies Dillen, Sebastian Munck, Jeroen Lammertyn, Dragana Spasic
Published in: Biosensors and Bioelectronics, Issue 152, 2020, Page(s) 112017, ISSN 0956-5663
DOI: 10.1016/j.bios.2020.112017

Microfluidic-controlled optical router for lab on a chip

Author(s): Jiri Dietvorst, Jeroen Goyvaerts, Tobias Nils Ackermann, Erica Alvarez, Xavier Muñoz-Berbel, Andreu Llobera
Published in: Lab on a Chip, Issue 19/12, 2019, Page(s) 2081-2088, ISSN 1473-0197
DOI: 10.1039/c9lc00143c

QCM mass underestimation in molecular biotechnology: Proximity ligation assay for norovirus detection as a case study

Author(s): Felix Neumann, Narayanan Madaboosi, Iván Hernández-Neuta, Jeanpierre Salas, Annika Ahlford, Vasile Mecea, Mats Nilsson
Published in: Sensors and Actuators B: Chemical, Issue 273, 2018, Page(s) 742-750, ISSN 0925-4005
DOI: 10.1016/j.snb.2018.06.025

Real-Time QCM Measurements of Rolling Circle Amplification Products

Author(s): Narayanan Madaboosi, Felix Neumann, Iván Hernández-Neuta, Jeanpierre Salas, Vasile Mecea, Mats Nilsson
Published in: Proceedings, Issue 1/4, 2017, Page(s) 509, ISSN 2504-3900
DOI: 10.3390/proceedings1040509

Asymptomatic bacteriuria in older adults: the most fragile women are prone to long-term colonization

Author(s): Michael Biggel, Stefan Heytens, Katrien Latour, Robin Bruyndonckx, Herman Goossens, Pieter Moons
Published in: BMC Geriatrics, Issue 19/1, 2019, ISSN 1471-2318
DOI: 10.1186/s12877-019-1181-4

Re-engineering 10–23 core DNA- and MNAzymes for applications at standard room temperature

Author(s): Karen Ven, Saba Safdar, Annelies Dillen, Jeroen Lammertyn, Dragana Spasic
Published in: Analytical and Bioanalytical Chemistry, Issue 411/1, 2019, Page(s) 205-215, ISSN 1618-2642
DOI: 10.1007/s00216-018-1429-4

Smartphone-based clinical diagnostics: towards democratization of evidence-based health care

Author(s): I. Hernández-Neuta, F. Neumann, J. Brightmeyer, T. Ba Tis, N. Madaboosi, Q. Wei, A. Ozcan, M. Nilsson
Published in: Journal of Internal Medicine, Issue 285/1, 2019, Page(s) 19-39, ISSN 0954-6820
DOI: 10.1111/joim.12820

The sweet detection of rolling circle amplification: Glucose-based electrochemical genosensor for the detection of viral nucleic acid

Author(s): Sibel Ciftci, Rocío Cánovas, Felix Neumann, Thomas Paulraj, Mats Nilsson, Gaston A. Crespo, Narayanan Madaboosi
Published in: Biosensors and Bioelectronics, Issue 151, 2020, Page(s) 112002, ISSN 0956-5663
DOI: 10.1016/j.bios.2019.112002

E-Beam Nanostructuring and Direct Click Biofunctionalization of Thiol–Ene Resist

Author(s): Reza Zandi Shafagh, Alexander Vastesson, Weijin Guo, Wouter van der Wijngaart, Tommy Haraldsson
Published in: ACS Nano, Issue 12/10, 2018, Page(s) 9940-9946, ISSN 1936-0851
DOI: 10.1021/acsnano.8b03709

High-Risk International Clones of Carbapenem-Nonsusceptible Pseudomonas aeruginosa Endemic to Indonesian Intensive Care Units: Impact of a Multifaceted Infection Control Intervention Analyzed at the Genomic Level

Author(s): Andreu Coello Pelegrin, Yulia Rosa Saharman, Aurélien Griffon, Mattia Palmieri, Caroline Mirande, Anis Karuniawati, Rudyanto Sedono, Dita Aditianingsih, Wil H. F. Goessens, Alex van Belkum, Henri A. Verbrugh, Corné H. W. Klaassen, Juliëtte A. Severin
Published in: mBio, Issue 10/6, 2019, ISSN 2150-7511
DOI: 10.1128/MBIO.02384-19

Phenotypic and Genomic Characterization of AmpC-Producing Klebsiella pneumoniae From Korea

Author(s): Mattia Palmieri, Stephane Schicklin, Andreu Coello Pelegrin, Sonia Chatellier, Christine Franceschi, Caroline Mirande, Yeon-Joon Park, Alex van Belkum
Published in: Annals of Laboratory Medicine, Issue 38/4, 2018, Page(s) 367, ISSN 2234-3806
DOI: 10.3343/alm.2018.38.4.367

Genomic Epidemiology of Carbapenem- and Colistin-Resistant Klebsiella pneumoniae Isolates From Serbia: Predominance of ST101 Strains Carrying a Novel OXA-48 Plasmid

Author(s): Mattia Palmieri, Marco Maria D’Andrea, Andreu Coello Pelegrin, Caroline Mirande, Snezana Brkic, Ivana Cirkovic, Herman Goossens, Gian Maria Rossolini, Alex van Belkum
Published in: Frontiers in Microbiology, Issue 11, 2020, ISSN 1664-302X
DOI: 10.3389/fmicb.2020.00294

Real-Time QCM Measurements of Rolling Circle Amplification Products

Author(s): Narayanan Madaboosi, Felix Neumann, Iván Hernández-Neuta, Jeanpierre Salas, Vasile Mecea and Mats Nilsson
Published in: Proceedings of eurosensors 2017, Issue 1(4), 2017, Page(s) 509

Modular Optofluidic Systems (MOPS)

Author(s): Tobias N. Ackermann; Jiri Dietvorst; Ana Sanchis; Juan P. Salvador; Xavier Munoz-Berbel; Erica Alvarez-Conde; Daniel Kopp; Hans Zappe; M.- Pilar Marco; Andreu Llobera;
Published in: Proceedings Volume 10013, SPIE BioPhotonics, 2016


Author(s): Guo, Weijin, Hansson, Jonas, van der Wijngaart, Wouter
Published in: Proceeding of 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS), IEEE, 2017, 2017

Viscosity Independent Paper Microfluidic Imbibition.

Author(s): Guo, W., Hansson, J., van der Wijngaart, W.
Published in: Proceedings of The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Issue MicroTAS 2016, 2016, Page(s) pp. 13-14

Formation of a thin-walled spider silk tube on a micromachined scaffold

Author(s): Weijin Guo, Linnea Gustafsson, Ronnie Jansson, My Hedhammar, Wouter van der Wijngaart
Published in: 2018 IEEE Micro Electro Mechanical Systems (MEMS), 2018, Page(s) 83-85
DOI: 10.1109/memsys.2018.8346488

Tuneable Microparticle Filters

Author(s): Emre Iseri, Kerem Kaya, Giacomo di Dio, Hiroki Yasuga, Norihisa Miki, Wouter van der Wijngaart
Published in: 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems (MEMS), 2019, Page(s) 290-291
DOI: 10.1109/memsys.2019.8870801

Droplet microfluidics inside paper

Author(s): Hiroki Yasuga, Weijin Guo, Jonas Hansson, Tommy Haraldsson, Norihisa Miki, Wouter van der Wijngaart
Published in: 2018 IEEE Micro Electro Mechanical Systems (MEMS), 2018, Page(s) 269-271
DOI: 10.1109/memsys.2018.8346536


Author(s): Guo, Weijin Hansson, Jonas van der Wijngaart, Wouter
Published in: Proceedings of The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2019, p. 1056-1057, 2019

Detection of E.coli in a digital assay

Author(s): Emre Iseri, Seref Akay, Wouter van der Wijngaart
Published in: 2018 IEEE Micro Electro Mechanical Systems (MEMS), 2018, Page(s) 301-303
DOI: 10.1109/memsys.2018.8346545

DNA-only bioassay for simultaneous detection of protein and nucleic acid targets on the self-powered iSIMPLE chip.

Author(s): Aida Montserrat, Saba Safdar, Karen Ven, Francesco Dal Dosso, Jeroen Lammertyn, Dragana Spasic
Published in: 2019

Stacking 2D Droplet Arrays for 3D Configurable Droplet Network

Author(s): Hiroki Yasuga, Toshihisa Osaki, Koki Kamiya, Emre Iseri, Wouter van der Wijngaart, Shoji Takeuchi, Norihisa Miki
Published in: 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems (MEMS), 2019, Page(s) 388-389
DOI: 10.1109/memsys.2019.8870863

Integrated Microwell Array Technologies for Single Cell Analysis

Author(s): Jolien Breukers, Caroline Struyfs, Sara Horta, Karin Thevissen, Karen Vanhoorelbeke, Bruno P. A. Cammue, Jeroen Lammertyn
Published in: Handbook of Single Cell Technologies, 2019, Page(s) 1-32
DOI: 10.1007/978-981-10-4857-9_21-1