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Digital readout of protein detection via super rolling circle amplification of DNA tags

Objective

This project will serve to evaluate market opportunities of a newly invented technology for digital detection of reporter DNA
strands in protein analysis.
The purpose of my ongoing ERC project ProteinSeq is to develop technology and reagents for parallel, sensitive and
specific targeted protein analyses in plasma samples, lysates, etc. An important limiting factor for the broader adoption of
our technology is the cost and throughput of next generation sequencing and realtime PCR to read out the identities and
levels of reporter DNA strands that reflect the amounts of proteins in a sample.
In the course of the ERC project we have invented and patented a technique to greatly enhance each of the reporter
strands to prominent DNA objects that can be easily detected, identified and counted by fluorescence microscopy with
automated image analysis or through flow cytometry. We refer to this highly specific technique as super rolling circle
amplification or sRCA, and it involves two generations of RCA and combinatorial fluorescence detection of the large
products, each composed of hundreds of thousand repeats of specific tag DNA sequences.
In the proposed PoC project we will position our new technology against other readout techniques such as next gen
sequencing, realtime PCR and microarrays by identifying Needs, Approach, Benefits, Competition for sRCA. Potential end
users will be contacted to establish customer requirements, and we will evaluate freedom to operate and the extent of
monopoly for our invention. We will also specifically investigate means to distinguish many sRCA products by combinatorial
labeling schemes.

Field of science

  • /natural sciences/biological sciences/genetics and heredity/dna
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins

Call for proposal

ERC-2015-PoC
See other projects for this call

Funding Scheme

ERC-POC - Proof of Concept Grant

Host institution

UPPSALA UNIVERSITET
Address
Von Kraemers Alle 4
751 05 Uppsala
Sweden
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 149 375

Beneficiaries (1)

UPPSALA UNIVERSITET
Sweden
EU contribution
€ 149 375
Address
Von Kraemers Alle 4
751 05 Uppsala
Activity type
Higher or Secondary Education Establishments