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Digital readout of protein detection via super rolling circle amplification of DNA tags

Objective

This project will serve to evaluate market opportunities of a newly invented technology for digital detection of reporter DNA
strands in protein analysis.
The purpose of my ongoing ERC project ProteinSeq is to develop technology and reagents for parallel, sensitive and
specific targeted protein analyses in plasma samples, lysates, etc. An important limiting factor for the broader adoption of
our technology is the cost and throughput of next generation sequencing and realtime PCR to read out the identities and
levels of reporter DNA strands that reflect the amounts of proteins in a sample.
In the course of the ERC project we have invented and patented a technique to greatly enhance each of the reporter
strands to prominent DNA objects that can be easily detected, identified and counted by fluorescence microscopy with
automated image analysis or through flow cytometry. We refer to this highly specific technique as super rolling circle
amplification or sRCA, and it involves two generations of RCA and combinatorial fluorescence detection of the large
products, each composed of hundreds of thousand repeats of specific tag DNA sequences.
In the proposed PoC project we will position our new technology against other readout techniques such as next gen
sequencing, realtime PCR and microarrays by identifying Needs, Approach, Benefits, Competition for sRCA. Potential end
users will be contacted to establish customer requirements, and we will evaluate freedom to operate and the extent of
monopoly for our invention. We will also specifically investigate means to distinguish many sRCA products by combinatorial
labeling schemes.

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

ERC-POC - Proof of Concept Grant

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) ERC-2015-PoC

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Host institution

UPPSALA UNIVERSITET
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 149 375,00
Address
VON KRAEMERS ALLE 4
751 05 Uppsala
Sweden

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Region
Östra Sverige Östra Mellansverige Uppsala län
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 149 375,00

Beneficiaries (1)

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