Periodic Reporting for period 4 - APPELS (A Probe of the Periodic Elements for Life in the Sea) Reporting period: 2020-12-01 to 2021-11-30 Summary of the context and overall objectives of the project The problem: Metals are a fundamental component of life. Biological processing of oxygen, nitrogen, sulphur and carbon all rely on metalloproteins. But less than half of all metalloproteins are characterised and the genome remains opaque to inference of metal binding and optimal concentrations for growth. APPELS aims to unveil, via cutting-edge techniques, a comprehensive view of the essential elements to life in the ocean, at the heart of the carbon cycle and global change, and will expand the dictionary of metal-binding motifs, applicable across the tree of life.The objectives:The overarching objective of APPELS is to probe the expanse of the periodic table to provide a comprehensive definition of the elements required by the modern marine phytoplankton (the metallome) and the marine metalloproteome. APPELS will revolutionise our understanding of metal-binding by proteins and the required elements that control the efficacy of biological pumping of carbon to depth in the ocean:• Explore which, as yet untested, elements are biologically important to 5 “model” marine phytoplankton, representative of contrasting marine chemotypes. Elements of interest include those suspected of importance (B, Si, V, Se, Cd, I), those thought to be of use to some species (Ti, Sn, Br) and those emerging from novel approaches to be part of life’s metallome (Sr, Ba, W, As, U, Pb, Ge);• At the physiological level, delineate the concentrations for maximal growth for all “suspected” elements, and identify any limitation implying requirement/use and the toxic threshold hence defining the “elemental sweet-spot”• Provide a complete elemental fingerprint of the metalloproteome of our 5 model organisms by coupling liquid chromatographic (LC) separation techniques with protein quantification and Quad- ICPMS elemental analysis,• Refine separation towards individual metalloproteins, particularly of unusual chemistry, to identify and characterise the interlinking between the selected metal, with coordination sites and protein function. Use combined peaks in protein abundance, and unusual element content to select cellularfractions worthy of refined separation to the individual protein level via LC, SDS- and nondenaturing PAGE gel. Identify the single proteins via high throughput tandem mass-spectrometry HT MS/MS with Mascot analysis• Assess concentration thresholds for metal competition and toxic interference (Cd, and Pb) within the metalloproteome by analysis of model organisms grown within a range of chemistries• Recombinant expression of novel metalloproteins within E. coli to confirm metal-selection at the active site, and to explore metal isotopic fractionation during enzyme assemblyImportance to Society:Trace metals lie at the heart of human health but are an underappreciated component of the human diet. Increasingly, getting the "right amount" of trace metals is being recognised as necessary for human health with elements such as in iron, selenium and iodine being identified as at most risk of deficiency whereas anthropogenically perturbed metals in the food chain such as arsenic, mercury and thallium pose a toxic risk. This ambitious, project aims to define the metallome and metalloproteome for marine life, ultimately the ancient ancestor of life on land and human beings. The probing of the farther reaches of the periodic table for biological use within APPELS will deliver an expansive definition of the metallome for marine life and the range of elemental concentrations that allow maximum growth of diverse phytoplankton chemotypes. It will pinpoint limiting and toxic thresholds for photosynthesisers, key to the chemical controls on the biological pump of atmospheric pCO2, and with implications for marine productivity in a future perturbed ocean and how elements will enter the marine foodchain. These “elemental sweetspots” allow powerful insight into the geological past in terms of how evolving chemistry dictated availability and adopted use of elements and controlled the past efficiency of the biological pump. The discovery of new essential elements for marine life, will unveil novel biochemistries with implications for higher organisms and optimal nutrition. Application of state-ofthe– art technologies to tackle the identity, and isotopic signature, of the metalloproteome will yield unrivalled insight into the relationship between those metals, their concentrations, and the associated proteins. This insight provides an enormous leap forwards in translating genetic codes to match proteins with their metal “pairs”, applicable across the tree of life. An ability to translate the genome to metal requirements opens the horizon for future identification of essential elements in other organisms from their genome, and chemical modelling of coordination site selectivity for metals, currently opaque to bioinformatics. Work performed from the beginning of the project to the end of the period covered by the report and main results achieved so far 1) We have developed and optimised a novel method for direct analysis of 32 elements simultaneously in small volume of cell lysate in buffers with a high salt matrix (800µL, up to 30% TDS). The method allows a high throughput analysis of complex samples (see Zhang et al., 2018: Direct measurement of multi-elements in high matrix samples with a flow injection ICP-MS: application to the extended Emiliania huxleyi Redfield ratio Q Zhang, JT Snow, P Holdship, D Price, P Watson, REM Rickaby, Journal of Analytical Atomic Spectrometry) which was presented as a poster at ICOBTE 2017, and in an invited talk in a Young Scientists Forum in Zhuhai, China. This has a wide application to multiple biological systems including humans. 2) A wide range of organisms (>15 species) have been analyzed for whole cell and intracellular elemental composition and can show the exotic chemistry that organisms accumulate when grown in a complex matrix of seawater chemistry. 3) We have identified 4 candidate metals for novel metalloproteins3) We have further worked towards compiling data for the ‘dose-response’ curves across the realm of marine microbes, thinking carefully about what a ‘metal requirement’ encapsulates in the context of phytoplankton and other marine microbes. The bulk of this has centred around iron (Fe), of which most data is available, compiling datasets of dose-response curves for a range of different microorganisms, and not just limited to photosynthetic microbes but also other marine microbes such as heterotrophic bacteria which have a key role in the carbon cycle. However, we have also began compiling the available data for Cu, Co and Zn. This compilation of data indicates that cellular metal quotas should be measured at the point where microorganisms just reach maximum growth – this prevents measurement of a value reflecting intracellular storage and instead reflects the true metallome and we are continuing to amass this data from the literature.4) We have been able to demonstrate an interaction between the nutrient status of a cell, and its susceptibility to metal toxicity. We have demonstrated a nutrient dependent toxicity where the toxic effects of V and As (both observed to accumulate in T.oceanica) are dependent on the cellular sufficiency of Phosphate. Under P deplete conditions the concentration at which both element becomes toxic is vastly lower than at P replete conditions. This is because there is competition between uptake of these chemically similar metals such that at low P availability, when the cell upregulates transport of P, there is enhanced mistaken uptake and cellular accumulation of V and As aggravating the toxicity within the cell. Progress beyond the state of the art and expected potential impact (including the socio-economic impact and the wider societal implications of the project so far) 1)) Ammonia-oxidising archaea (AOA) mediate the rate limiting step of nitrification, the central component of the marine nitrogen cycle that converts ammonia to nitrite then nitrate. Competition with phytoplankton for ammonium and light inhibition are considered to restrict AOA activity to below the photic zone, but observations of surface nitrification now demand a further understanding of the factors driving AOA distribution and activity. Pico- to nanomolar concentrations of iron (Fe) limit the growth of microorganisms in a significant portion of the world’s surface oceans, yet there is no examination of the role of Fe in AOA growth despite the process of ammonia oxidation being considered to rely on the micronutrient. Here we investigate the Fe requirements and Fe uptake strategies of the Nitrosopumilus maritimus strain SCM1, a strain representative of globally abundant marine AOA. Using trace metal clean culturing techniques, we found that N. maritimus growth is controlled by Fe availability, displaying a free inorganic Fe (Fe´) half saturation constant 1-2 orders of magnitude greater for cell growth than numerous marine phytoplankton and heterotrophic bacteria driven by a reduced affinity for Fe´. In addition we discovered that, whilst unable to produce siderophores to enhance access to Fe, N. maritimus is able to use the exogenous siderophore desferrioxamine B (DFB), likely through a reductive uptake pathway analogous to that demonstrated in phytoplankton. Our work suggests AOA growth in surface waters may be Fe limited and advances our understanding of AOA physiology at the cellular level with implications for ecosystem dynamics and the biogeochemical N-cycle. This work is under review at ISME journal. 2) We have found that Cr, U, Ni and Tl can promote growth of some microorganisms when added beyond current environmental concentrations, elements normally considered toxic at such high concentrations. These elements are our candidates to separate and identify the proteins which bind these metals and find novel biological use in the coming years of the project. 3) At the Palaeozoic-Mesozoic boundary, the dominance of marine eukaryotic algae shifted from the green (chlorophyll b) to the red (chlorophyll c) superfamily. Selection pressures caused by the bioavailability of trace metals associated with an increasing oxygenation of the ocean may have played a key role in this algal revolution. From a broad scan of elemental compositions, a significant difference in the cellular Cr/P quota was found between the two superfamilies. The different responses to Cr exposure reveal contrasting strategies for metal uptake and homeostasis in these algal lineages. At high Cr(VI) concentrations, red lineages experience growth inhibition through reduced photosynthetic capability, while green lineages are completely unaffected. Moreover, Cr(VI) has a more significant impact on the metallomes of red lineage algae, in which metal/P ratios increased with increasing Cr(VI) concentration for many trace elements. Green algae have higher specificity transporters to prevent Cr(VI) from entering the cell, and more specific intracellular stores of Cr within the membrane fraction than the red algae, which accumulate more Cr mistakenly in the cytosol fraction via lower affinity transport mechanisms. Green algal approaches require greater nutrient investments in the more numerous transport proteins required and management of specific metals, a strategy better adapted to the resource-rich coastal waters. By contrast the red algae are nutrient efficient with fewer and less discriminate metal transporters which can be fast and better adapted in the oligotrophic, oxygenated open ocean which has prevailed since the deepening of the oxygen minimum zones at the start of the Mesozoic. This work is in revision for publication in Limnology and Oceanography.4) Thallium (Tl) is the most toxic metal of all and is present at concentrations of concern over a wide geographical area. The roles of biology in the Tl cycle have seldom been considered. Here we present dose-response curves and physiological data from a range of species of phytoplankton under a wide range of Tl concentrations (1 ng/L to 1 mg/L), spanning modern marine concentrations. Of all phytoplankton studied here, the cyanobacteria Synechococcus and the haptophyte P. granifera have the largest tolerance to Tl toxicity. Furthermore in each of these species, growth has even been stimulated at high Tl concentration (1mg/L) beyond the natural concentration range. Haptophytes, especially isochrysidales, significantly accumulated more Tl intracellularly than chlorophytes did. Potassium channels of various phytoplankton, the likely main route of mistaken Tl entry into cells, have been identified from their genome using a novel orthogroup inference algorithm and provided a framework to understand the bioaccumulation of Tl. The potassium transporters from E. huxleyi are significantly different from all other phytoplankton in this study. Indeed, E. huxleyi accumulated most Tl available in the media. Based on the data in this study, under average Tl concentration in seawater (10 ng/L), E. huxleyi can accumulate ~1.6 µg Tl per g organic C produced by this organism. Such high levels of accumulation in the marine primary producers has significant implications for bioaccumulation up the marine food chain and the levels of toxins in the human diet. This work is currently under preparation for submission.Perhaps the biggest outcome of our work to date is that the metal stoichiometry of cells in terms of cell quotas is not fixed and does not reflect biological use of metals. Many of the elements accumulated by cells are transported by mistake rather than on purpose and they get held in the cell purely as part of the homeostatic mechanisms. As a result, we are directing our efforts towards understanding the different metal acquisition and homeostatic mechanisms expressed via the transporters of the cell. We are starting to determine potential differences in metal transportation strategies by different algal superfamilies, which may be the reason for their different physiological responses to trace elements concentrations in the environment, their differing ecology in the environment, and may even explain the different mineralisation potential of cells. The extended Redfield ratio of the ubiquitous coccolithophore, Emiliania huxleyi.