Periodic Reporting for period 3 - BrainBIT (All-optical brain-to-brain behaviour and information transfer)
Reporting period: 2019-12-01 to 2021-05-31
In this project, we setup and characterize new optical imaging and manipulation techniques to address brain functionality over the large scale.
In addition, we characterized several red-shifted genetically engineered calcium indicators with large-scale cortical imaging in awake mice. To perform a full-optical imaging and transfer of neuronal activity, we chose these new calcium indicators with reduced optical cross-talk with the most commonly used neuronal actuators.
In Montagni et al., 2018 we describe the evaluation of different indicators of neuronal activity to identify the most suited for the development of an all-optical system as stated in the WP2 T2.1 of the project. The problem addressed concern the identification of the best red-shifted indicator to reveal the cortical neuronal activity. To this aim, we tested different red-shifted genetically encoded calcium indicators. The overall objective is to identify a combination of indicator and actuator that could be successfully used to read and write from mouse cortex, towards the intra and inter subject transfer of neuronal functionality. In the paper by Montagni et al., we identified jRCaMP1a as the best red-shifted functional indicator that does not show photoswitch, resulting the best choice for the future development of an all-optical system for interrogation of neuronal circuits.
In Sancataldo et al. (Front. Neuroanat. 2019), we described the use of acousto-optic deflectors (AODs) in light-sheet microscopy to mitigate striping artefacts. This work paves the way to a more quantitative functional imaging in zebrafish larvae, which is a key aspect of the project, and which was also previously investigated by our group (Muellenbroich et al., Front. Cell Neurosci. 2018). Further, the use of AODs has been a technical training for the implementation of multiplexed ultrafast light-sheet microscopy, described previously in the report.