Periodic Reporting for period 2 - MERA (Mechanism of Enzyme Rhodopsin Activation)
Reporting period: 2018-04-01 to 2019-09-30
Our goal is the in depth characterization of Rhodopsin-cyclases by application of a number of biophysical and biophysical techniques. Our intention is to dissect the signal transduction pathway into a number of molecular events starting from the light absorption to the full activation of the protein and the detailed reaction steps of catalysis during GTP to cGMP conversion.
Enzyme rhodopsins and in particular Rhodopsin-cyclases will be used for Optogenetic applications. This means the modulation of the important cellular second messengers cGMP and cAMP non-invasively with light. This could be of great relevance for the neurosciences as well as for cell biology and clinical research.
The overall objective is a detailed molecular and mechanistic understanding of rhodopsin cyclases as unique examples of membrane bound photoactivated enzymes.