Periodic Reporting for period 3 - CDK6-DrugOpp (CDK6 in transcription - turning a foe in a friend)
Reporting period: 2019-09-01 to 2021-02-28
To allow the detailed study of CDK6 in leukemic stem cells we have generated novel cellular tools. Hematopoietic stem cells are on top of the hierarchy of the blood system. A few stem cells suffice to generate the entire blood flowing through our body. This limited cell numbers make it particularly difficult to study these cells. We have succeeded in generated a hematopoietic stem/progenitor cell line that can replenish the entire blood system in a mouse. This novel cell lines can also be used to generate different versions of leukemic stem cells that drive leukemia formation. These novel leukemic stem cell lines faithfully recapitulate human blood cancer in mice and will serve as valuable novel tool to understand the molecular mechanisms of cellular transformation underlying blood cancer.
We have also started to unravel how DK6 drives transformation and which features of the molecule is needed to drive transcription.