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Exponential Amplification and Rapid Detection of miRNAs using DNA-Quantum Dot Bioconjugates for Disease Diagnostics

Objective

Accurate and timely diagnosis is critical in the treatment of all diseases. Researchers are constantly discovering new links between specific biomolecules and diseases, often uncovering ‘panels’ of markers with high sensitivity and specificity for disease diagnostics. However, our current diagnostic tools are unable to take advantage of these panels as they lack sensitivity, or are too costly and slow for practical application. Accordingly, new technologies that can help transform these fundamental discoveries into revolutionary clinical tools are in high demand. These must be rapid, efficient, robust and cost-effective. To this end, I will develop a new rapid diagnostic assay that combines the unique functional properties of fluorescent nanomaterials and enzymes. The core technology is an advanced nanoparticle-biomolecule construct consisting of highly fluorescent ‘quantum dot’ inorganic nanoparticles coated with DNA. These nanobioconjugates (termed ‘AmpiDots’) act simultaneously as both templates for isothermal amplification of target miRNAs and as highly sensitive fluorescent sensors. The result will be a rapid, highly sensitive, versatile and efficient assay that can detect multiple targets simultaneously and give a robust signal for assay readout. A critical aspect of the work will be the use of advanced microfluidic-based analytical systems to optimise the assay and to study the unique phenomena that arise when enzymes interact with nanoparticles.

Fields of science (EuroSciVoc)

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Programme(s)

Multi-annual funding programmes that define the EU’s priorities for research and innovation.

Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MSCA-IF-EF-ST - Standard EF

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2015

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Coordinator

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 187 419,60
Address
Raemistrasse 101
8092 Zuerich
Switzerland

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Region
Schweiz/Suisse/Svizzera Zürich Zürich
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 187 419,60
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