MicroRNAs are short nucleotide sequences that regulate expression of specific target genes, and can be found in bodily fluids such as plasma. We compared the expression profile of hundreds of miRNAs in plasma samples from male and female children with CF and analysed these expression profiles for correlations with various parameters such as CFTR genotype, age, lung infection status and antibiotic treatment. We found that miR-885-5p levels were significantly increased in the female samples. We then performed bioinformatic gene ontology and pathway analysis of miR-885-5p validated target genes in order to gain insight into the possible biological significance of miR-885-5p within the context of CF progression. RAC1-mediated signaling was the most dominant result from these analyses which may have wide-ranging implications in CF, including its role in enhancing the rescue of cystic fibrosis transmembrane conductance regulator (CFTR) to the cell surface membrane in concert with pharmacological correctors. Other pathways of relevance include cell migration/motility, inflammation and fibrosis.