Periodic Reporting for period 3 - CrackEpitranscriptom (Cracking the epitranscriptome)
Reporting period: 2019-11-01 to 2021-04-30
In our proposal, we set to explore the roles of a particularly abundant modification called m6A, involving the methylation of an adenosine. We are studying this modification in the context of a specific environmental response in yeast called meiosis, during which we have previously found that m6A is induced across >1000 mRNA molecules. We have three key goals: First, to identify unknown components that are involved in mRNA methylation, and to identify specific m6A sites that are important for meiosis. Second, to understand the function of mRNA methylation, by looking at the molecular consequences to having versus not having methylation. Third, to understand how such functional roles are mediated.
A second leap forward is our ability to generate large pools of designed mutants varying in their methylation levels in a programmable way. This allows us to to obtain much more direct relationships between the methylation and distinct, functional readouts that we can obtain.
These two leaps in our technological toolkit will allow us to now address the functions and mechanisms of action of m6A at unprecedented resolution and by the end of this project we hope to be able to make major breakthroughs into these questions.