Periodic Reporting for period 3 - MultiSense (Multisensory signal processing: From brain-wide neuronal circuits to behavior)
Reporting period: 2020-03-01 to 2021-08-31
Second, we gained high control over the visual environment of the fish by developing a two-photon version of the system based on an infrared light-sheet for which the fish is blind so that the laser light-sheet does not excite the photoreceptors in the fish eye. The challenge was to fiber deliver the femtosecond pulsed laser required for two-photon microscopy onto our rotating light-sheet platform. We achieved broadband and dispersion free laser delivery with a hollow-core negative curvature fiber. This fiber should find additional application in head mounted miniscopes for the excitation of a large spectrum of optogenetic and fluorescent proteins via a single fiber or to share the high cost laser source required for two-photon microscopy between different setups. With our rotatable two-photon system, we succeeded to mapped, for the first time, the pure brain-wide vestibular response of larval zebrafish to dynamic tilt stimuli (Trentesaux et al. manuscript in preparation).