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Synthetic bacterial lipid rafts to optimize industrial bioprocesses

Objective

Genetic tractability of bacterial cells allows generating synthetic microbial chassis platforms (SMCPs) with remarkable biotechnological applications but their functionality currently faces important off-genome limitations due to deficient protein-protein interactions, unfavorable protein stoichiometry or generation of toxic intermediates that ultimately compromise the industrial production processes. To solve this problem, Rafts4Biotech project will take advantage of our recent discovery, that bacteria are able to organize subcellular membrane compartments similar to the so-called lipid rafts of eukaryotic cells, to improve/protect specific cellular processes. Rafts4Biotech project will engineer bacterial cells to confine biotechnologically relevant reactions into bacterial lipid rafts to optimize their stoichiometry and protect cells from undesirable metabolic interferences. Hence, the Rafts4Biotech project will produce new generation reliable and robust SMCPs in which industrial production processes are confined in bacterial lipid rafts, released from their classical off-genome limitations and optimized for industrial production. Moreover, this concept can be applied to many prokaryotes, since lipid rafts happens to occur in many bacterial species. Based on this versatility, Raft4Biotech project will use two biotechnologically relevant biosystems, Bacillus subtilis and Escherichia coli, to engineer synthetic bacterial lipid rafts to optimize the performance of three challenging biochemical processes in the fields of pharmaceutical, cosmetics and feed industrial sectors. To achieve this, Rafts4Biotech consortium combines different expertise in synthetic biology, systems biology and mathematical modeling and it includes a number of SMEs that will actively work in this project and will translate this technology into market application. The technology developed by Rafts4Biotec will optimize multistep industrial processes and invigorate European research.

Field of science

  • /social sciences/economics and business/business and management/commerce
  • /natural sciences/mathematics/applied mathematics/mathematical model
  • /natural sciences/biological sciences/microbiology/bacteriology
  • /natural sciences/biological sciences/synthetic biology
  • /social sciences/economics and business/economics/production economics
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/proteomics

Call for proposal

H2020-NMBP-BIO-2016
See other projects for this call

Funding Scheme

RIA - Research and Innovation action

Coordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS
Address
Calle Serrano 117
28006 Madrid
Spain
Activity type
Research Organisations
EU contribution
€ 1 569 778,75

Participants (11)

FAKULTNI NEMOCNICE U SV. ANNY V BRNE
Czechia
EU contribution
€ 600 007,50
Address
Pekarska 53
656 91 Brno
Activity type
Higher or Secondary Education Establishments
GEORG-AUGUST-UNIVERSITAT GOTTINGENSTIFTUNG OFFENTLICHEN RECHTS
Germany
EU contribution
€ 700 000
Address
Wilhelmsplatz 1
37073 Gottingen
Activity type
Higher or Secondary Education Establishments
INSTITUT PASTEUR
France
EU contribution
€ 600 250
Address
Rue Du Docteur Roux 25-28
75724 Paris Cedex 15
Activity type
Research Organisations
RIJKSUNIVERSITEIT GRONINGEN
Netherlands
EU contribution
€ 613 706,25
Address
Broerstraat 5
9712CP Groningen
Activity type
Higher or Secondary Education Establishments
ENGENES BIOTECH GMBH
Austria
EU contribution
€ 434 450
Address
Mooslackengasse 17
1190 Wien
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
UNIVERSITATSMEDIZIN GREIFSWALD KORPERSCHAFT DES OFFENTLICHEN RECHTS
Germany
EU contribution
€ 446 250
Address
Fleischmannstrasse 8
17489 Greifswald
Activity type
Higher or Secondary Education Establishments
EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Switzerland
EU contribution
€ 0
Address
Raemistrasse 101
8092 Zuerich
Activity type
Higher or Secondary Education Establishments
NAICONS SRL
Italy
EU contribution
€ 555 000
Address
Via Guaragna 3
20147 Saronno Va
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
BIOSYNTIA APS
Denmark
EU contribution
€ 555 250
Address
Kogle Alle 6
2970 Horsholm
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
ENANTIS SRO
Czechia
EU contribution
€ 369 250
Address
Kamenice 771/34
625 00 Brno
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
SCIENSEED SL
Spain
EU contribution
€ 289 637,50
Address
Calle Arte 3 5/A
28033 Madrid
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)