Educational Network in Ocular Drug Delivery and Therapeutics

Project Information

OcuTher

Grant agreement ID: 722717

DOI 10.3030/722717

Project closed

EC signature date 16 August 2016

Start date 1 November 2016

End date 31 October 2020

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost € 3 933 728,88

EU contribution € 3 933 728,88

Coordinated by

ITA-SUOMEN YLIOPISTO
Finland

Deliverables

Other (2)

Call for applications
Awarding of doctoral degrees

Websites, patent fillings, videos etc. (1)

First online version of network public website

Publications

Peer reviewed articles (34)

Insight into N-terminal localization and dynamics of engineered virus-like particles

Author(s): Daan F. M. Vervoort, Chiara Pretto, Jan C. M. van Hest
Published in: RSC Advances, Issue 10/64, 2020, Page(s) 38774-38781, ISSN 2046-2069
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0ra07612k

Oxidative Stress and Mitochondrial Damage in Dry Age-Related Macular Degeneration Like <i>NFE2L2/PGC-1α</i> <i>^-/-</i> Mouse Model Evoke Complement Component C5a Independent of C3

Author(s): Iswariyaraja Sridevi Gurubaran; Hanna Heloterä; Stephen Marry; Ali Koskela; Juha M. T. Hyttinen; Jussi J. Paterno; Arto Urtti; Mei Chen; Heping Xu; Anu Kauppinen; Kai Kaarniranta
Published in: Biology, 2021, ISSN 2079-7737
Publisher: MDPI
DOI: 10.3390/biology10070622

Topical ocular pharmacokinetics and bioavailability for a cocktail of atenolol, timolol and betaxolol in rabbits

Author(s): Anam Fayyaz, Veli-Pekka Ranta, Elisa Toropainen, Kati-Sisko Vellonen, Annika Valtari, Jooseppi Puranen, Marika Ruponen, Iain Gardner, Arto Urtti, Masoud Jamei, Eva M. del Amo
Published in: European Journal of Pharmaceutical Sciences, Issue 155, 2020, Page(s) 105553, ISSN 0928-0987
Publisher: Elsevier BV
DOI: 10.1016/j.ejps.2020.105553

Versatile Reversible Cross-Linking Strategy to Stabilize CCMV Virus Like Particles for Efficient siRNA Delivery

Author(s): Chiara Pretto, Jan C. M. van Hest
Published in: Bioconjugate Chemistry, Issue 30/12, 2019, Page(s) 3069-3077, ISSN 1043-1802
Publisher: American Chemical Society
DOI: 10.1021/acs.bioconjchem.9b00731

Pullulan Based Bioconjugates for Ocular Dexamethasone Delivery

Author(s): Eva Kicková; Stefano Salmaso; Francesca Mastrotto; Paolo Caliceti; Arto Urtti
Published in: Pharmaceutics, 2020, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics13060791

Modulation of three key innate immune pathways for the most common retinal degenerative diseases

Author(s): Isha Akhtar‐Schäfer, Luping Wang, Tim U Krohne, Heping Xu, Thomas Langmann
Published in: EMBO Molecular Medicine, Issue 10/10, 2018, Page(s) e8259, ISSN 1757-4676
Publisher: John Wiley & Sons Ltd.
DOI: 10.15252/emmm.201708259

Carboxylesterase Activities and Protein Expression in Rabbit and Pig Ocular Tissues

Author(s): Anam Hammid, John K Fallon, Toni Lassila, Giulia Salluce, Philip C Smith, Ari Tolonen, Achim Sauer, Arto Urtti, Paavo Honkakoski
Published in: Mol Pharm, Issue 18(3), 2021, Page(s) 1305-1316, ISSN 1543-8384
Publisher: American Chemical Society
DOI: 10.1021/acs.molpharmaceut.0c01154

Release of functional dexamethasone by intracellular enzymes: A modular peptide-based strategy for ocular drug delivery

Author(s): Madhushree Bhattacharya, Amir Sadeghi, Sanjay Sarkhel, Marja Hagström, Sina Bahrpeyma, Elisa Toropainen, Seppo Auriola, Arto Urtti
Published in: Journal of Controlled Release, Issue 327, 2020, Page(s) 584-594, ISSN 0168-3659
Publisher: Elsevier BV
DOI: 10.1016/j.jconrel.2020.09.005

A technical protocol for an experimental ex vivo model using arterially perfused porcine eyes

Author(s): C. Rousou, P. Hoogenboom, K.A. van Overdam, G. Storm, J. Dorrestijn, E. Mastrobattista
Published in: Experimental Eye Research, Issue 181, 2019, Page(s) 171-177, ISSN 0014-4835
Publisher: Academic Press
DOI: 10.1016/j.exer.2019.02.003

Dexamethasone Loaded Liposomes by Thin-Film Hydration and Microfluidic Procedures: Formulation Challenges

Author(s): MD Al-Amin, Federica Bellato, Francesca Mastrotto, Mariangela Garofalo, Alessio Malfanti, Stefano Salmaso, Paolo Caliceti
Published in: International Journal of Molecular Sciences, Issue 21/5, 2020, Page(s) 1611, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms21051611

Fatty acids and oxidized lipoproteins contribute to autophagy and innate immunity responses upon the degeneration of retinal pigment epithelium and development of age-related macular degeneration

Author(s): Kai Kaarniranta, Ali Koskela, Szabolcs Felszeghy, Niko Kivinen, Antero Salminen, Anu Kauppinen
Published in: Biochimie, Issue 159, 2019, Page(s) 49-54, ISSN 0300-9084
Publisher: Elsevier BV
DOI: 10.1016/j.biochi.2018.07.010

Pharmacokinetics of intravitreal macromolecules: scaling between rats and rabbits

Author(s): Amir Sadeghi, Jooseppi Puranen, Marika Ruponen, Annika Valtari, Astrid Subrizi, Veli-Pekka Ranta, Elisa Toropainen, Arto Urtti
Published in: European Journal of Pharmaceutical Sciences, 2021, Page(s) 105720, ISSN 0928-0987
Publisher: Elsevier BV
DOI: 10.1016/j.ejps.2021.105720

Pharmacokinetics of Pullulan–Dexamethasone Conjugates in Retinal Drug Delivery

Author(s): Eva Kicková, Amir Sadeghi, Jooseppi Puranen, Shirin Tavakoli, Merve Sen, Veli-Pekka Ranta, Blanca Arango-Gonzalez, Silvia Bolz, Marius Ueffing, Stefano Salmaso, Paolo Caliceti, Elisa Toropainen, Marika Ruponen, Arto Urtti
Published in: Pharmaceutics, 2022, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics14010012

Ultrasound and Microbubbles for the Treatment of Ocular Diseases: From Preclinical Research towards Clinical Application

Author(s): Charis Rousou; Carl C. L. Schuurmans; Arto Urtti; Enrico Mastrobattista; Gert Storm; Chrit Moonen; Kai Kaarniranta; Roel Deckers
Published in: Pharmaceutics, Issue 2, 2021, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics13111782

Advances in Drug Delivery and Biomaterials: Facts and Vision

Author(s): Paolo Caliceti, Pietro Matricardi
Published in: Pharmaceutics, Issue 11/1, 2019, Page(s) 48, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics11010048

Retinal neuroprotection by controlled release of a VCP inhibitor from self-assembled nanoparticles

Author(s): Merve Sen, Md Al-Amin, Eva Kicková, Amir Sadeghi, Jooseppi Puranen, Arto Urtti, Paolo Caliceti, Stefano Salmaso, Blanca Arango-Gonzalez, Marius Ueffing
Published in: J Control Release, 2021, ISSN 0168-3659
Publisher: Elsevier BV
DOI: 10.1016/j.jconrel.2021.09.039

Ocular pharmacokinetics of atenolol, timolol and betaxolol cocktail: Tissue exposures in the rabbit eye

Author(s): Anam Fayyaz, Kati-Sisko Vellonen, Veli-Pekka Ranta, Elisa Toropainen, Mika Reinisalo, Annika Valtari, Jooseppi Puranen, Giuseppe D'Amico Ricci, Emma M. Heikkinen, Iain Gardner, Marika Ruponen, Arto Urtti, Masoud Jamei, Eva M. del Amo
Published in: European Journal of Pharmaceutics and Biopharmaceutics, Issue 166, 2021, Page(s) 155-162, ISSN 0939-6411
Publisher: Elsevier BV
DOI: 10.1016/j.ejpb.2021.06.003

Mitophagy in the Retinal Pigment Epithelium of Dry Age-Related Macular Degeneration Investigated in the NFE2L2/PGC-1α-/- Mouse Model

Author(s): Iswariyaraja Sridevi Gurubaran, Johanna Viiri, Ali Koskela, Juha M.T. Hyttinen, Jussi J. Paterno, Gréta Kis, Miklós Antal, Arto Urtti, Anu Kauppinen, Szabolcs Felszeghy, Kai Kaarniranta
Published in: International Journal of Molecular Sciences, Issue 21/6, 2020, Page(s) 1976, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms21061976

Mechanistical retinal drug targets and challenges

Author(s): Kai Kaarniranta, Heping Xu, Anu Kauppinen
Published in: Advanced Drug Delivery Reviews, Issue 126, 2018, Page(s) 177-184, ISSN 0169-409X
Publisher: Elsevier BV
DOI: 10.1016/j.addr.2018.04.016

Magnetically Assisted Drug Delivery of Topical Eye Drops Maintains Retinal Function In Vivo in Mice

Author(s): Marco Bassetto, Daniel Ajoy, Florent Poulhes, Cathy Obringer, AurelieWalter, Nadia Messadeq, Amir Sadeghi, Jooseppi Puranen, Marika Ruponen, Mikko Kettunen, Elisa Toropainen, Arto Urtti, Hélène Dollfus, Olivier Zelphati, and Vincent Marion
Published in: Pharmaceutics, 2021, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics13101650

Intravitreal hydrogels for sustained release of therapeutic proteins

Author(s): Blessing C. Ilochonwu, Arto Urtti, Wim E. Hennink, Tina Vermonden
Published in: Journal of Controlled Release, Issue 326, 2020, Page(s) 419-441, ISSN 0168-3659
Publisher: Elsevier BV
DOI: 10.1016/j.jconrel.2020.07.031

In vivo phenotypic and molecular characterization of retinal degeneration in mouse models of three ciliopathies

Author(s): Agnès Brun, Xiangxiang Yu, Cathy Obringer, Daniel Ajoy, Elodie Haser, Corinne Stoetzel, Michel J. Roux, Nadia Messaddeq, Hélène Dollfus, Vincent Marion
Published in: Experimental Eye Research, Issue 186, 2019, Page(s) 107721, ISSN 0014-4835
Publisher: Academic Press
DOI: 10.1016/j.exer.2019.107721

Cytokine Signaling Protein 3 Deficiency in Myeloid Cells Promotes Retinal Degeneration and Angiogenesis through Arginase-1 Up-Regulation in Experimental Autoimmune Uveoretinitis

Author(s): Mei Chen, Jiawu Zhao, Imran H.A. Ali, Stephen Marry, Josy Augustine, Mohajeet Bhuckory, Aisling Lynch, Adrien Kissenpfennig, Heping Xu
Published in: The American Journal of Pathology, Issue 188/4, 2018, Page(s) 1007-1020, ISSN 0002-9440
Publisher: American Society for Investigative Pathology
DOI: 10.1016/j.ajpath.2017.12.021

Single‐cell RNA sequencing study of retinal immune regulators identified CD47 and CD59a expression in photoreceptors—Implications in subretinal immune regulation

Author(s): Jian Liu, Miao Tang, Kevin Harkin, Xuan Du, Chang Luo, Mei Chen, Heping Xu
Published in: Journal of Neuroscience Research, 2020, ISSN 0360-4012
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/jnr.24618

Microplasma assisted synthesis of gold nanoparticle/graphene oxide nanocomposites and their potential application in SERS sensing

Author(s): Daye Sun, Miao Tang, Li Zhang, Brian G Falzon, Dilli Babu Padmanaban, Davide Mariotti, Paul Maguire, Heping Xu, Mei Chen, Dan Sun
Published in: Nanotechnology, Issue 30/45, 2019, Page(s) 455603, ISSN 0957-4484
Publisher: Institute of Physics Publishing
DOI: 10.1088/1361-6528/ab2a23

Characterization, Stability, and In Vivo Efficacy Studies of Recombinant Human CNTF and Its Permeation into the Neural Retina in Ex Vivo Organotypic Retinal Explant Culture Models

Author(s): Jaakko Itkonen, Ada Annala, Shirin Tavakoli, Blanca Arango-Gonzalez, Marius Ueffing, Elisa Toropainen, Marika Ruponen, Marco G. Casteleijn, Arto Urtti
Published in: Pharmaceutics, Issue 12/7, 2020, Page(s) 611, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics12070611

Design and synthesis of lipid-mimetic cationic iridium complexes and their liposomal formulation for in vitro and in vivo application in luminescent bioimaging

Author(s): Julia R. Shakirova, Amir Sadeghi, Alla A. Koblova, Pavel S. Chelushkin, Elisa Toropainen, Shirin Tavakoli, Leena-Stiina Kontturi, Tatu Lajunen, Sergey P. Tunik, Arto Urtti
Published in: RSC Advances, Issue 10/24, 2020, Page(s) 14431-14440, ISSN 2046-2069
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0ra01114b

Macrophage to myofibroblast transition contributes to subretinal fibrosis secondary to neovascular age-related macular degeneration

Author(s): Karis Little; Maria Llorián-Salvador; Miao Tang; Xuan Du; Stephen Marry; Mei Chen; Heping Xu
Published in: Journal of Neuroinflammation, Issue 17:355, 2020, ISSN 1742-2094
Publisher: BioMed Central
DOI: 10.1186/s12974-020-02033-7

Ocular metabolism and distribution of drugs in the rabbit eye: Quantitative assessment after intracameral and intravitreal administrations

Author(s): Eva M Del Amo, Anam Hammid, Melanie Tausch, Elisa Toropainen, Amir Sadeghi, Annika Valtari, Jooseppi Puranen, Mika Reinisalo, Marika Ruponen, Arto Urtti, Achim Sauer, Paavo Honkakoski
Published in: International Journal of Pharmacy and Pharmaceutical Sciences, Issue 613, 2021, ISSN 0378-5173
Publisher: Elsevier BV
DOI: 10.1016/j.ijpharm.2021.121361

New Method for Efficient siRNA Delivery in Retina Explants: Reverse Magnetofection

Author(s): Marco Bassetto, Merve Sen, Florent Poulhes, Blanca Arango-Gonzalez, Elise Bonvin, Cedric Sapet, Marius Ueffing, and Olivier Zelphati
Published in: Bioconjugate Chemistry, 2021, ISSN 1043-1802
Publisher: American Chemical Society
DOI: 10.1021/acs.bioconjchem.1c00132

Efficient Ocular Delivery of VCP siRNA via Reverse Magnetofection in RHO P23H Rodent Retina Explants

Author(s): Merve Sen; Marco Bassetto; Florent Poulhes; Olivier Zelphati; Marius Ueffing; Blanca Arango-Gonzalez
Published in: Pharmaceutics, 2021, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics13020225

Cowpea Chlorotic Mottle Virus‐Like Particles as Potential Platform for Antisense Oligonucleotide Delivery in Posterior Segment Ocular Diseases

Author(s): Chiara Pretto; Miao Tang; Mei Chen; Heping Xu; Astrid Subrizi; Arto Urtti; Jan C. M. Hest
Published in: Macromolecular Bioscience, 21(8):2100095. Wiley-VCH Verlag, Issue 4, 2021, ISSN 1616-5195
Publisher: Wiley-VCH GmbH
DOI: 10.1002/mabi.202100095

Ocular Intracameral Pharmacokinetics for a Cocktail of Timolol, Betaxolol, and Atenolol in Rabbits

Author(s): Anam Fayyaz, Veli-Pekka Ranta, Elisa Toropainen, Kati-Sisko Vellonen, Giuseppe D’Amico Ricci, Mika Reinisalo, Emma M. Heikkinen, Iain Gardner, Arto Urtti, Masoud Jamei, Eva M. del Amo
Published in: Molecular Pharmaceutics, 2020, Page(s) 588–594, ISSN 1543-8384
Publisher: American Chemical Society
DOI: 10.1021/acs.molpharmaceut.9b01024

Intravitreal Polymeric Nanocarriers with Long Ocular Retention and Targeted Delivery to the Retina and Optic Nerve Head Region

Author(s): Vijayabhaskarreddy Junnuthula, Amir Sadeghi Boroujeni, Shoupeng Cao, Shirin Tavakoli, Roxane Ridolfo, Elisa Toropainen, Marika Ruponen, Jan C M van Hest, Arto Urtti
Published in: Pharmaceutics, 2021, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics13040445

Conference proceedings (3)

Inhibition of VCP structurally and functionally reconstitutes photoreceptor cells in RhP23H organotypic retina cultures

Author(s): Blanca Arango-Gonzalez; Merve Sen; Tsui-Fen Chou; Ray Deshaies; Sylvia Bolz; Wadood Haq; Marius Ueffing
Published in: Investigative Ophthalmology & Visual Science, Issue 60,4878, 2019
Publisher: ARVO Journals
DOI: 10.5281/zenodo.4485697

Pharmacological modulation of the VCP/ERAD/proteasome axis rescues photoreceptor degeneration in P23H rhodopsin

Author(s): Merve Sen, Oksana Kutsyr, Sylvia Bolz, Tsui‐Fen Chou, Ray Deshaies, Blanca Arango Gonzalez and Marius Ueffing
Published in: Acta Ophthalmologica, Issue 97,S263, 2019
Publisher: Wiley Online Library
DOI: 10.1111/j.1755-3768.2019.5383

Pharmacological interference in the VCP/ERAD/proteasome axis rescues photoreceptor degeneration in RhP23H

Author(s): Merve Sen; Oksana Kutsyr; Sylvia Bolz; Tsui-Fen Chou; Ray Deshaies; Blanca Arango-Gonzalez and Marius Ueffing
Published in: Investigative Ophthalmology & Visual Science, Issue 60,4881, 2019
Publisher: ARVO Journals
DOI: 10.5281/zenodo.4485790

Non-peer reviewed articles (2)

Synthesis and application of magnetic nanoparticles (MNPs) in siRNA delivery to the retina

Author(s): Marco Bassetto, Merve Sen, Blanca Arango Gonzalez, Marius Ueffing, Florent Poulhes, Olivier Zelphati
Published in: Acta Ophthalmologica, Issue 97/S263, 2019, ISSN 1755-375X
Publisher: Wiley-Blackwell
DOI: 10.1111/j.1755-3768.2019.5378

Synthesis and application of magnetic nanoparticles (MNPs) in drug delivery to the retina

Author(s): Marco Bassetto, Daniel Ajoy Moreno, Florent Poulhes, Vincent Marion, Helene Dollfus, Olivier Zelphati
Published in: Acta Ophthalmologica, Issue 97/S263, 2019, ISSN 1755-375X
Publisher: Wiley-Blackwell
DOI: 10.1111/j.1755-3768.2019.5380