Periodic Reporting for period 3 - SUBDECODE (Dendritic encoding of movement in space)
Reporting period: 2020-01-01 to 2021-06-30
understanding the rules that define the output of a neuron from identified input patterns.
Our main goals are (1) to understand how different inputs contribute to neuronal output firing of subicular neurons. The subiculum is a brain region at the interface between the hippocampal formation, a brain region important for learning and memroy processes, and the neocortex. It generates an important hippocampal output code that is interpreted by other brain regions. Moreover (2), we aim at monitoring free behavior at unprecedented detail to investigate how behavioral modules contribute to dendritic integration on the single cell level. Finally (3) we want to use behavioral observation exclusively to predict the membrane potential fluctuations of subicular neurons.
we use several experimental steps to build a detailed, data-driven biophysical model of subicular neurons. This general model will be the basis for the generation of cell-type specific models during the optimization process. It is based on our data on somatic and dendritic electrophysiological properties and two-photon imaging of dendritic and somatic activity during head-fixed and free behavior. Our main goal is to generate a model that can use dense behavioral information to generate a prediction of neuronal output, which we test against experimental data using in-vivo single cell (patch clamp) recordings. Using this approach we aim at generating computational models of subicular neurons that are capable of processing realistic spatially tuned inputs. With the knowledge of the movement trajectory and head direction of the animal these models may allow an estimation of the membrane potential of single subicular pyramidal cells during an unconstrained behaviour.