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Capturing non-Amplified Tumor Circulating DNA with Ultrasound Hydrodynamics

Deliverables

Website construction

Specific objectives, main actions and their results will be disseminated through a website, designed to have two parts: one publicly accessible, where the translation of project’s results into clinical practice will be advertised, and a second one, password protected, for partners’ communication only.

Targeted NGS analysis on the tissue samples of the 200 patients

In the case of solid tumours, formalin fixed paraffin embedded (FFPE) blocks are usually the only tumor material available. Although the DNA obtained from FFPE is highly fragmented and cross-linked due to fixation, it has been demonstrated to yield NGS results comparable to those of fresh frozen tissue, as long as appropriate protocols are employed.21 Based on its long experience, the Lab. of Tumor Biology at the UOC will ensure robust analysis of the DNA from FFPE blocks. The mutations identified by NGS will also be confirmed by alternative methods depending on the type of alteration to be validated. P4 is in close collaboration with the Medical Oncology Dept., Univ. Hospital of Heraklion and runs mutation tests for diagnostic purposes in more than 200 samples per year.

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Publications

3D-printed Point-of-Care Platform for Genetic Testing of Infectious Diseases Directly in Human Samples Using Acoustic Sensors and a Smartphone

Author(s): George Papadakis, Alexandros K. Pantazis, Maria Ntogka, Konstantinos Parasyris, Gesthimani-Ioanna Theodosi, Georgia Kaprou, Electra Gizeli
Published in: ACS Sensors, Issue 4/5, 2019, Page(s) 1329-1336, ISSN 2379-3694
DOI: 10.1021/acssensors.9b00264

Microfluidic extraction and digital quantification of circulating cell-free DNA from serum

Author(s): Karla Perez-Toralla, Iago Pereiro, Sonia Garrigou, Fahima Di Federico, Charlotte Proudhon, François-Clément Bidard, Jean-Louis Viovy, Valérie Taly, Stéphanie Descroix
Published in: Sensors and Actuators B: Chemical, Issue 286, 2019, Page(s) 533-539, ISSN 0925-4005
DOI: 10.1016/j.snb.2019.01.159