Periodic Reporting for period 1 - Vanrestrep (Vancomycin resistance regulation in the antibiotic-producers streptomycetes)
Reporting period: 2018-02-01 to 2020-01-31
Part of this work has been published in the gold open access journal BMC Genomics. The work is entitled as follows: “Genome sequencing analysis of Streptomyces coelicolor mutants that overcome the phosphate-depending vancomycin lethal effect”. The work has been also disseminated as a communication to the International conference on “Bacterial Persistence and Antimicrobial Therapy” organized by EMBO in 2018 in Ascona (Switzerland). Other part of this work is in preparation for publication and the results have been already presented at the 14th International Symposium on the Genetics of Industrial Microorganisms held in Pisa (Italy) in September 2019.
The results of this project has brought to light evidences with regards to the nutritional regulation of the antibiotic resistance mechanism of certain bacteria and consequently of the protocols for testing antimicrobial resistance. Antimicrobial susceptibility testing (AST) is clinically used to guide antimicrobial therapy. Disk-diffusion and broth or agar dilution assays are the standard methods to evaluate the in vitro antimicrobial activity of a compound. However, as shown in this project, inorganic phosphate has a strong impact in the final results of these AST methods when testing the activity of certain extracts or pure compounds such as vancomycin. Therefore, new media compositions that are not sensitive to the changes in inorganic phosphate should be developed and patented. In this sense, the work carried out in this project is in the process of a patent filling on new process of AST methods and it has been already selected by the Innovation Radar initiative, supported by the European Commission, as an innovative work under the title: “New media additive for antimicrobial susceptibility testing”.