Encapsulated eukaryotic ribosome assembly

Project Information

Glowsome

Grant agreement ID: 741781

DOI 10.3030/741781

Project closed

EC signature date 30 June 2017

Start date 1 September 2017

End date 31 August 2022

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 000 000,00

EU contribution € 2 000 000,00

Coordinated by

RUPRECHT-KARLS-UNIVERSITAET HEIDELBERG
Germany

Publications

Non-peer reviewed articles (5)

A Homologous Recombination System to Generate Epitope-Tagged Target Genes in Chaetomium thermophilum: A Genetic Approach to Investigate Native Thermostable Proteins

Author(s): Nikola Kellner, Sabine Griesel, Ed Hurt
Published in: International Journal of Molecular Sciences, Issue 23 (6), 2022, Page(s) 3198, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23063198

Global Transcriptome Characterization and Assembly of the Thermophilic Ascomycete Chaetomium thermophilum

Author(s): Amit Singh, Géza Schermann, Sven Reislöhner, Nikola Kellner, Ed Hurt, Michael Brunner
Published in: Genes, Issue 12, 2021, Page(s) 1549, ISSN 2073-4425
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes12101549

Eukaryotic Ribosome Assembly

Author(s): Jochen Baßler, Ed Hurt
Published in: Annual Review of Biochemistry, Issue 88/1, 2019, Page(s) 281-306, ISSN 0066-4154
Publisher: Annual Reviews, Inc.
DOI: 10.1146/annurev-biochem-013118-110817

Emergence of the primordial pre-60S from the 90S pre-ribosome

Author(s): Sherif Ismail, Dirk Flemming, Matthias Thoms, Jose Vincente Gomes-Filho, Lennart Randau, Roland Beckmann, Ed Hurt
Published in: Cell Reports, Issue 39(1), 2022, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2022.110640

Structure of the Maturing 90S Pre-ribosome in Association with the RNA Exosome

Author(s): Benjamin Lau, Jingdong Cheng, Dirk Flemming, Giuseppe La Venuta, Otto Berninghausen, Roland Beckmann, Ed Hurt
Published in: Mol Cell, Issue 81(2), 2021, Page(s) 293-303.e4., ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2020.11.009

Peer reviewed articles (10)

In vitro structural maturation of an early stage pre-40S particle coupled with U3 snoRNA release and central pseudoknot formation

Author(s): Jingdong Cheng, Giuseppe La Venuta, Benjamin Lau, Otto Berninghausen, Roland Beckmann, Ed Hurt
Published in: Nucleic Acids Research, Issue Vol. 50, No. 20, 2022, Page(s) 11916-11923, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkac910

Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release

Author(s): Benjamin Lau, Olga Beine-Golovchuk, Markus Kornprobst, Jingdong Cheng, Dieter Kressler, Beáta Jády, Tamás Kiss, Roland Beckmann, Ed Hurt
Published in: Cell Reports, Issue 2022 Nov 22;41(8):111684, 2022, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2022.111684

90S pre-ribosome transformation into the primordial 40S subunit

Author(s): Jingdong Cheng , Benjamin Lau, Giuseppe La Venuta, M. Ameismeier, Otto Berninghausen, Ed Hurt, Roland Beckmann
Published in: Science, Issue 00368075, 2020, Page(s) 1470-1476, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.abb4119

Mpp10 represents a platform for the interaction of multiple factors within the 90S pre-ribosome

Author(s): Bebiana Sá-Moura, Markus Kornprobst, Satyavati Kharde, Yasar Luqman Ahmed, Gunter Stier, Ruth Kunze, Irmgard Sinning, Ed Hurt
Published in: PLOS ONE, Issue 12/8, 2017, Page(s) e0183272, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0183272

3.2-Å-resolution structure of the 90S preribosome before A1 pre-rRNA cleavage

Author(s): Jingdong Cheng, Nikola Kellner, Otto Berninghausen, Ed Hurt, Roland Beckmann
Published in: Nature Structural & Molecular Biology, Issue 24/11, 2017, Page(s) 954-964, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/nsmb.3476

Interdependent action of KH domain proteins Krr1 and Dim2 drive the 40S platform assembly

Author(s): Miriam Sturm, Jingdong Cheng, Jochen Baßler, Roland Beckmann, Ed Hurt
Published in: Nature Communications, Issue 8/1, 2017, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-017-02199-4

Eukaryotic ribosome assembly, transport and quality control

Author(s): Cohue Peña, Ed Hurt, Vikram Govind Panse
Published in: Nature Structural & Molecular Biology, Issue 24/9, 2017, Page(s) 689-699, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/nsmb.3454

Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration

Author(s): Jingdong Cheng, Jochen Baßler, Paulina Fischer, Benjamin Lau, Nikola Kellner, Ruth Kunze, Sabine Griesel, Martina Kallas, Otto Berninghausen, Daniela Strauss, Roland Beckmann, Ed Hurt
Published in: Molecular Cell, Issue 75/6, 2019, Page(s) 1256-1269.e7, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2019.06.032

A Puzzle of Life: Crafting Ribosomal Subunits

Author(s): Dieter Kressler, Ed Hurt, Jochen Baßler
Published in: Trends in Biochemical Sciences, Issue 42/8, 2017, Page(s) 640-654, ISSN 0968-0004
Publisher: Elsevier BV
DOI: 10.1016/j.tibs.2017.05.005

The nucleoplasmic phase of pre-40S formation prior to nuclear export

Author(s): Jingdong Chen, Benjamin Lau, Matthias Thoms, Michael Ameismeier, Otto Berninghausen, Ed Hurt, Roland Beckmann
Published in: Nucleic Acids Research, Issue Vol. 50, No. 20, 2022, Page(s) 11924–11937, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkac961

Book chapters (1)

Transformation of Chaetomium thermophilum and affinity-purification of Native tTermostable Protein Complexes

Author(s): Nikola Kellner, Ed Hurt
Published in: Methods in Molecular Biology, Issue 2502, 2022, Page(s) 35-50, ISBN 978-1-0716-2337-4
Publisher: Springer Nature
DOI: 10.1007/978-1-0716-2337-4_2