Mimicking liver disease and regeneration in vitro for drug development and liver transplantation

Project Information

HEPASPHER

Grant agreement ID: 742020

DOI 10.3030/742020

Project closed

EC signature date 15 June 2017

Start date 1 September 2017

End date 30 June 2023

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 413 449,00

EU contribution € 2 413 449,00

Coordinated by

KAROLINSKA INSTITUTET
Sweden

Publications

Peer reviewed articles (18)

Prediction of Drug-Induced Hepatotoxicity Using Long-Term Stable Primary Hepatic 3D Spheroid Cultures in Chemically Defined Conditions

Author(s): Sabine U Vorrink, Yitian Zhou, Magnus Ingelman-Sundberg, Volker M Lauschke
Published in: Toxicological Sciences, Issue 163/2, 2018, Page(s) 655-665, ISSN 1096-6080
Publisher: Oxford University Press
DOI: 10.1093/toxsci/kfy058

3D primary hepatocyte culture systems for analyses of liver diseases, drug metabolism and toxicity: Emerging culture paradigms and applications

Author(s): Volker M. Lauschke, Reza Zandi Shafagh, Delilah F. G. Hendriks, Magnus Ingelman‐Sundberg
Published in: Biotechnology Journal, 2019, Page(s) 1800347, ISSN 1860-6768
Publisher: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/biot.201800347

Comparison of Hepatic 2D Sandwich Cultures and 3D Spheroids for Long-term Toxicity Applications: A Multicenter Study

Author(s): Catherine C Bell, Anita C A Dankers, Volker M Lauschke, Rowena Sison-Young, Roz Jenkins, Cliff Rowe, Chris E Goldring, Kevin Park, Sophie L Regan, Tracy Walker, Chris Schofield, Audrey Baze, Alison J Foster, Dominic P Williams, Amy W M van de Ven, Frank Jacobs, Jos van Houdt, Tuula Lähteenmäki, Jan Snoeys, Satu Juhila, Lysiane Richert, Magnus Ingelman-Sundberg
Published in: Toxicological Sciences, Issue 162/2, 2018, Page(s) 655-666, ISSN 1096-6080
Publisher: Oxford University Press
DOI: 10.1093/toxsci/kfx289

AMP-activated protein kinase activation and NADPH oxidase inhibition by inorganic nitrate and nitrite prevent liver steatosis

Author(s): Isabel Cordero-Herrera, Mikael Kozyra, Zhengbing Zhuge, Sarah McCann Haworth, Chiara Moretti, Maria Peleli, Mayara Caldeira-Dias, Arghavan Jahandideh, Han Huirong, Josiane de Campos Cruz, Andrei L. Kleschyov, Marcelo F. Montenegro, Magnus Ingelman-Sundberg, Eddie Weitzberg, Jon O. Lundberg, Mattias Carlstrom
Published in: Proceedings of the National Academy of Sciences, Issue 116/1, 2019, Page(s) 217-226, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1809406115

Novel genetic and epigenetic factors of importance for inter-individual differences in drug disposition, response and toxicity

Author(s): Volker M. Lauschke, Yitian Zhou, Magnus Ingelman-Sundberg
Published in: Pharmacology & Therapeutics, 2019, ISSN 0163-7258
Publisher: Pergamon Press
DOI: 10.1016/j.pharmthera.2019.01.002

Human Liver Spheroids as a Model to Study Aetiology and Treatment of Hepatic Fibrosis

Author(s): Tracey Hurrell, Vlasia Kastrinou-Lampou, Achilleas Fardellas, Delilah F. G. Hendriks, Åsa Nordling, Inger Johansson, Audrey Baze, Céline Parmentier, Lysiane Richert, Magnus Ingelman-Sundberg
Published in: Cells, Issue 9/4, 2020, Page(s) 964, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells9040964

Managing the challenge of drug-induced liver injury: a roadmap for the development and deployment of preclinical predictive models

Author(s): Richard J. Weaver, Eric A. Blomme, Amy E. Chadwick, Ian M. Copple, Helga H. J. Gerets, Christopher E. Goldring, Andre Guillouzo, Philip G. Hewitt, Magnus Ingelman-Sundberg, Klaus Gjervig Jensen, Satu Juhila, Ursula Klingmüller, Gilles Labbe, Michael J. Liguori, Cerys A. Lovatt, Paul Morgan, Dean J. Naisbitt, Raymond H. H. Pieters, Jan Snoeys, Bob van de Water, Dominic P. Williams, B. Kevin Park
Published in: Nature Reviews Drug Discovery, Issue 19/2, 2020, Page(s) 131-148, ISSN 1474-1776
Publisher: Nature Publishing Group
DOI: 10.1038/s41573-019-0048-x

Impact of CYP2C19 genotype on sertraline exposure in 1200 Scandinavian patients

Author(s): Line S. Bråten, Tore Haslemo, Marin M. Jukic, Magnus Ingelman-Sundberg, Espen Molden, Marianne K. Kringen
Published in: Neuropsychopharmacology, Issue 45/3, 2020, Page(s) 570-576, ISSN 0893-133X
Publisher: Nature Publishing Group
DOI: 10.1038/s41386-019-0554-x

CYP3A5 is unlikely to mediate anticancer drug resistance in hepatocellular carcinoma

Author(s): Volker M Lauschke, Åsa Nordling, Yitian Zhou, Sara Fontalva, Isabel Barragan, Magnus Ingelman-Sundberg
Published in: Pharmacogenomics, Issue 20/15, 2019, Page(s) 1085-1092, ISSN 1462-2416
Publisher: Ashley Publications Ltd.
DOI: 10.2217/pgs-2019-0094

Mechanisms of Chronic Fialuridine Hepatotoxicity as Revealed in Primary Human Hepatocyte Spheroids

Author(s): Delilah F G Hendriks, Tracey Hurrell, Julia Riede, Muriëlle van der Horst, Sarianna Tuovinen, Magnus Ingelman-Sundberg
Published in: Toxicological Sciences, Issue 171/2, 2019, Page(s) 385-395, ISSN 1096-6080
Publisher: Oxford University Press
DOI: 10.1093/toxsci/kfz195

Calcium Signaling in Liver Injury and Regeneration

Author(s): Nuria Oliva-Vilarnau, Simona Hankeova, Sabine U. Vorrink, Souren Mkrtchian, Emma R. Andersson, Volker M. Lauschke
Published in: Frontiers in Medicine, Issue 5, 2018, ISSN 2296-858X
Publisher: Springer
DOI: 10.3389/fmed.2018.00192

The TM6SF2 E167K genetic variant induces lipid biosynthesis and reduces apolipoprotein B secretion in human hepatic 3D spheroids

Author(s): Sebastian Prill, Andrea Caddeo, Guido Baselli, Oveis Jamialahmadi, Paola Dongiovanni, Raffaela Rametta, Kajsa P. Kanebratt, Arturo Pujia, Piero Pingitore, Rosellina Margherita Mancina, Daniel Lindén, Carl Whatling, Annika Janefeldt, Mikael Kozyra, Magnus Ingelman-Sundberg, Luca Valenti, Tommy B. Andersson, Stefano Romeo
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-47737-w

Three-Dimensional Spheroid Primary Human Hepatocytes in Monoculture and Coculture with Nonparenchymal Cells

Author(s): Audrey Baze, Céline Parmentier, Delilah F.G. Hendriks, Tracey Hurrell, Bruno Heyd, Philippe Bachellier, Catherine Schuster, Magnus Ingelman-Sundberg, Lysiane Richert
Published in: Tissue Engineering Part C: Methods, Issue 24/9, 2018, Page(s) 534-545, ISSN 1937-3384
Publisher: Mary Ann Liebert Inc.
DOI: 10.1089/ten.tec.2018.0134

Inter-individual differences in the susceptibility of primary human hepatocytes towards drug-induced cholestasis are compound and time dependent

Author(s): Céline Parmentier, Delilah F.G. Hendriks, Bruno Heyd, Philippe Bachellier, Magnus Ingelman-Sundberg, Lysiane Richert
Published in: Toxicology Letters, Issue 295, 2018, Page(s) 187-194, ISSN 0378-4274
Publisher: Elsevier BV
DOI: 10.1016/j.toxlet.2018.06.1069

Characterisation of the NRF2 transcriptional network and its response to chemical insult in primary human hepatocytes: implications for prediction of drug-induced liver injury

Author(s): Ian M. Copple, Wouter den Hollander, Giulia Callegaro, Fiona E. Mutter, James L. Maggs, Amy L. Schofield, Lucille Rainbow, Yongxiang Fang, Jeffrey J. Sutherland, Ewa C. Ellis, Magnus Ingelman-Sundberg, Stephen W. Fenwick, Christopher E. Goldring, Bob van de Water, James L. Stevens, B. Kevin Park
Published in: Archives of Toxicology, Issue 93/2, 2019, Page(s) 385-399, ISSN 0340-5761
Publisher: Springer Verlag
DOI: 10.1007/s00204-018-2354-1

Clinically relevant CYP3A4 induction mechanisms and drug screening in 3D spheroid cultures of primary human hepatocytes

Author(s): Delilah F.G. Hendriks, Sabine U. Vorrink, Tomas Smutny, Sarah C. Sim, Åsa Nordling, Shahid Ullah, Masaki Kumondai, Barry C. Jones, Inger Johansson, Tommy B. Andersson, Volker M. Lauschke, Magnus Ingelman‐Sundberg
Published in: Clinical Pharmacology & Therapeutics, 2020, ISSN 0009-9236
Publisher: Nature Publishing Group
DOI: 10.1002/cpt.1860

Human hepatic 3D spheroids as a model for steatosis and insulin resistance

Author(s): Mikael Kozyra, Inger Johansson, Åsa Nordling, Shahid Ullah, Volker M. Lauschke, Magnus Ingelman-Sundberg
Published in: Scientific Reports, Issue 8/1, 2018, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-018-32722-6

Prediction of drug response and adverse drug reactions: From twin studies to Next Generation Sequencing

Author(s): Volker M. Lauschke, Magnus Ingelman-Sundberg
Published in: European Journal of Pharmaceutical Sciences, Issue 130, 2019, Page(s) 65-77, ISSN 0928-0987
Publisher: Elsevier BV
DOI: 10.1016/j.ejps.2019.01.024