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Widening gene pool of bread wheat by hybridization with Aegilops biuncialis supported by advanced genetic and chromosome genomic approaches

Objective

The widening of gene pool of bread wheat (Triticum aestivum) by employing its wild relatives as donors of new alleles and genes will ensure the ability of wheat to sustain yield quality and quantity under adverse environmental conditions. Species from genus Aegilops represent a rich source of agronomically important traits, such as leaf rust resistance, grain nutritional quality and abiotic stress tolerance. To date, the utilization of wild genetic diversity has been hampered by low throughput of methods used to select introgression lines and the lack of knowledge on the genomes of wild relatives.
The multidisciplinary AEGILWHEAT project integrates the applicant’s expertise in interspecific hybridization and cytomolecular genome analysis of Aegilops species with the state of art expertise in flow-cytometric sorting and genomic analysis of plant mitotic chromosomes in the Centre of Plant structural and Functional genomics (IEB, Olomouc, CZ). The aims of this project are to (1) isolate individual U and M chromosomes from Aegilops biuncialis and (2) identify their gene content and produce polymorphic markers (SNPs, INDELs) by next-generation sequencing and bioinformatic analyses. The genes and new markers will be integrated into (3) a new high resolution genetic linkage map of Ae. biuncialis. The marker-assisted selection system based on the ordered markers will be used for the (4) high throughput selection of wheat-Ae. biuncialis introgression lines.
The training through the research will extend the expertise of the applicant by new skills in molecular genetics, genomics and bioinformatics so that he can apply innovative genomics approaches to speed up his wheat pre-breeding programmes in Hungary. The project will deliver valuable information to geneticists and breeders on chromosome structure and genome evolution within the tribe Triticeae and provide molecular tools to support cost-effective transfer of wild alleles into wheat.

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

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Funding Scheme

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MSCA-IF-EF-ST - Standard EF

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2016

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Coordinator

USTAV EXPERIMENTALNI BOTANIKY AV CR
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 154 720,80
Address
ROZVOJOVA 263
165 02 PRAHA 6
Czechia

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Region
Česko Praha Hlavní město Praha
Activity type
Research Organisations
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 154 720,80
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