Objective
The inner working of many fundamental biological nanomachines implies many stochastic changes in conformation and molecular interactions. These changes are reflected as variability in the activity of the same molecule over time, and in heterogeneous activities between different molecules. By the direct observation at the molecular scale of these nanomachines activity, single-molecule fluorescence techniques have gained access to this stochastic information, which is otherwise missed by bulk techniques but is essential for their understanding. However, the widespread use of these techniques in many biological systems has been hampered by the low working concentration (nM) determined by the diffraction limit of light, and current nanophotonic solutions to this problem are technically too demanding. Here, we propose self-assembled DNA origami nanoantennas as nanophotonic platforms aiming to break this concentration barrier by means of fluorescence signal enhancing and reduction of the observation volume. The versatility of DNA origami structures, biocompatibility and ability for site-directed immobilization of biomolecules, make them perfectly suited to perform complex biological assays. In the presented action we aim to achieve single-molecule fluorescence DNA sequencing using DNA origami nanoantennas to probe the potential of these platforms to perform complex bioassays, in the high concentration regime and demanding multiplexing. Moreover, since they avoid the fabrication and instrumental challenges related to other nanophotonic devices, self-assembly DNA origami nanoantennas are amenable, easy to handle and friendly technology to the biological experimenter, and thus we expect to boost their use in biology. Besides, the fulfilment of this action will provide the fellow with a complete formative training that would boost his future scientific career, and will generate also a new DNA-sequencing technology that will impact positively European scientific excellence.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology materials engineering colors
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules
- engineering and technology nanotechnology nano-materials
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2016
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
80539 MUNCHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.