Objective
Background
DNA hydroxymethylation and methylation are marks on DNA that help define cell identity and maintain genome stability. DNA hydroxymethylation is recently discovered, and the mechanisms underlying its maintenance are uncharacterised. DNA methylation is crucial for cell function, but large blocks of DNA lose methylation in cancerous and ageing cells. It has been speculated that this is due to aberrant maintenance during cell division, however, technical limitations have prevented this from being directly assessed. By developing a novel technology to study maintenance of these marks, I will test this hypothesis for the first time.
Approach
This new technology will track how DNA methylation and hydroxymethylation patterns are restored after DNA replication, using both mass-spectrometry and genomics. Using this, I will track restoration of these marks following DNA replication, and test whether DNA methylation loss is caused by cell cycle speed, depleted methionine levels late in replication, or a combination of both. This will be followed up by functional analyses of key maintenance DNA hydroxymethylation and methylation factors. Thus, this work combines my past experience in DNA methylation with the host lab’s expertise in chromatin dynamics during DNA replication.
Impact
This will be the first quantitative study of how patterns of DNA methylation and DNA hydroxymethylation are propagated between cell divisions, which is essential to their roles in defining and maintaining cell identity. The results will bring seminal and novel understanding of these marks from both basic and biomedical perspectives, by elucidating how epigenome maintenance is linked to both DNA replication and the epigenetic changes seen in disease. By dissecting in unprecedented resolution the mechanisms underlying propagation of DNA methylation and hydroxymethylation, this work will unveil the basis for epigenetic inheritance of these marks between cell generations.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2016
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
1165 KOBENHAVN
Denmark
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.