Objective
Symbiotic nitrogen fixation by rhizobia takes place in specialized organs of legume host plants, the root nodules. While rather deep molecular insights into plant-rhizobia recognition, early nodule organogenesis, regulation of nodulation and nitrogen fixation are available, much less is known about how nodule integrity is maintained and the origin of the underlying morphogenetic program. Recently, NOOT BOP COCH LIKE (NBCL) genes of Medicago truncatula and Pisum sativum were found to ensure nodule integrity by repressing ectopic root formation (applicant's former project). Interestingly, soybean nodules elicited by a Bradyrhizobium diazoefficiens mutant lacking the general stress response sigma factor σEcfG also formed ectopic roots (host's project), pointing to a bacteria-plant signalling system that is crucial for nodule persistence and integrity. Here, we propose to decipher the molecular determinants that maintain determinate nodule identity using the B. diazoefficiens – soybean model. We will combine plant molecular genetics, cell biology with transcriptomics and metabolomics to unravel cells, genes and metabolic networks that contribute to the checkpoint system ensuring nodule integrity. In work package 1 (WP1), the role of three soybean NBCL orthologs will be unravelled. In WP2, the nodule-to-root conversion characteristic for B. diazoefficiens ΔecfG mutants will be studied at the cellular level using root tip-specific reporter fusions. In WP3, a combination of metabolomics and transcriptomics will be applied to identify metabolites and gene networks involved in the nodule-to-root conversion elicited by ΔecfG and nbcl mutants. Altogether, results of this project will provide insight into determinants and mechanisms used by the economically important group of soybean plants to maintain the integrity of specialized, root-derived organs, an aspect that is relevant also in the light of ongoing attempts to engineer non-legumes into rhizobial hosts.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences molecular biology molecular genetics
- natural sciences biological sciences cell biology
- natural sciences biological sciences biological behavioural sciences ethology biological interactions
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds legumes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2016
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
8092 Zuerich
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.