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Minimal Model for Pox-Virus Infection

Objective

Viral diseases represent one of the world’s highest socio-economic burdens. Increased global trade and travel, climate change resulting in shifting viral vectors, and the emergence of new and often deadly viruses is inevitable. Therefore, detailed understanding of the complexity of virus particles and the development of new model systems to study them, will be essential to develop new research, diagnostic, and therapeutic tools. The structural changes that occur during the initial contact between a virus and its host remains one of the major challenges in infection biology. Until recently, the investigation of viral nano-architecture and dynamic changes that occur in virus particles during the infectious lifecycle was limited to methods, such as EM, with no capacity to capture dynamic events or define molecular specificity. The goal of the proposed project is to create a new minimal model of virus infection based on cell-derived membrane blebs. The model will be amenable to novel super-resolution microscopy (SRM) methods that allow the visualization of viral structures at resolutions of tens of nanometers. Recently developed analytical tools like single-virion averaging allows the generation of precise models from hundreds of events. This affords unprecedented insights into the biological and biophysical requirements of virus infection. Furthermore, we aim to investigate the dynamics of virus architectural changes during the first stages of infection, particularly at the membrane level, by combining single-molecule techniques with our new model-system. While initially aimed at investigating protein structure-function relationships within the prototypic poxvirus, vaccinia, the model system and imaging developments outlined will be broadly applicable to a wide range of biological systems including other viruses. Thereby, this proposal looks to advance the field of infection biology into the nanoscale.

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MSCA-IF-EF-ST - Standard EF

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2016

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Coordinator

UNIVERSITY COLLEGE LONDON
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 183 454,80
Address
GOWER STREET
WC1E 6BT LONDON
United Kingdom

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Region
London Inner London — West Camden and City of London
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 183 454,80
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