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Epigenetically Stable Cloned Plants: Increasing The Epigenetic and Phenotypic Diversity of Plants through Tissue-Specific Regeneration

Objective

Most of phenotypic variation comes from variation in DNA sequence. However, a number of studies have clearly shown that phenotype variation and diversity can also be the result of epigenetic changes. The major goal of this proposal is to develop a novel methodology for robust production of epigenetically stable plant variants, in order to increase epigenetic and phenotypic diversity. A key element in the project will be a recent breakthrough in the generation of clonal individuals from different tissue types using inducible overexpression of the embryonic regulatory protein RKD4. Preliminary work shows that plants regenerated from roots inherit many aspects of root specific methylation and gene expression patterns, even in the leaf tissue. Importantly, these features are stably transmitted across generations. For this project, I propose to: (1) evaluate physiological consequences of altered epigenomes in RKD4 regenerated plants; (2) compare (epi)genomic and phenotypic variation between plants regenerated using hormones and RKD4 overexpression; (3) compare epigenomic and phenotypic consequences in regenerants of different Arabidopsis thaliana accessions and close A. thaliana relatives Arabidopsis lyrata and Capsella rubella. The program, which will be carried out under supervision of Detlef Weigel, a world leader in plant genetics and genomics, will substantially contribute to increasing the toolkit for plant breeders to generate meaningful phenotypic diversity.

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MSCA-IF-EF-ST - Standard EF

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) H2020-MSCA-IF-2016

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Coordinator

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 171 460,80
Address
HOFGARTENSTRASSE 8
80539 MUNCHEN
Germany

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Region
Bayern Oberbayern München, Kreisfreie Stadt
Activity type
Research Organisations
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 171 460,80
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