Objective
Cilia are highly conserved eukaryotic organelles that, with the exception of fungi and higher plants are present in nearly all types of organisms from protists to mammals. Through their motility, sensory, and signaling functions cilia play crucial roles in human physiology and development. Defects in cilia have profound impact on human health. Cilia assembly requires a dedicated protein shuttle, intraflagellar transport (IFT), a bidirectional motility of multi-megadalton protein arrays along ciliary microtubules. Although the overall mechanism of transport is generally described, complete high resolution structural description is necessary for the detailed understanding of this complex system.
I propose the in-depth structural analysis of IFT features, train types, cargoes, interactions with the molecular motors in the cilia model organism Chlamydomonas. This approach will bring us both a molecular resolution of incorporated protein complexes and dynamic overview of their relations.
The project objectives will be achieved by an elabrated chain of methods based on correlative microscopy, combining the specificity of nanobody labelling with the structure preservation and molecular resolution of cryo-electron tomography. We plan to develop two independent workflows for tracing the IFT features based on the most progressive cryo methods in the cooperation with the project partners.
It is anticipated that an detailed ultrastructural 3D analysis of intraflagellar transport will provide new fundamental mechanistic insights into this very important biological process. We also expect an overlap to the whole structural biology field due to the methodical development, which will be universally applicable for another structural studies.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences data science
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy electron microscopy
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- natural sciences biological sciences molecular biology structural biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2016
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
80539 MUNCHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.