Deciphering virus-host interactions using correlated confocal-atomic force microscopy

Project Information

NanoVirus

Grant agreement ID: 758224

DOI 10.3030/758224

Project closed

EC signature date 25 September 2017

Start date 1 January 2018

End date 31 December 2022

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 1 998 125,00

EU contribution € 1 998 125,00

Coordinated by

UNIVERSITE CATHOLIQUE DE LOUVAIN
Belgium

Publications

Peer reviewed articles (16)

Reovirus directly engages integrin to recruit clathrin for entry into host cells

Author(s): M. Koehler, S.J.L. Petitjean, J. Yang, P. Aravamudhan, X. Somoulay, C. Lo Giudice, M.A. Poncin, A.C. Dumitru, T.S. Dermody, D. Alsteens
Published in: Nature Communications, Issue 20411723, 2021, Page(s) 2149, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-22380-0

Stepwise Enzymatic-Dependent Mechanism of Ebola Virus Binding to Cell Surface Receptors Monitored by AFM

Author(s): Qingrong Zhang; Jinsung Yang; Sueli Tillieux; Zhengyuan Guo; Rita dos Santos Natividade; Melanie Koehler; Simon Petitjean; Zongqiang Cui; David Alsteens
Published in: Nano Letters, Vol. 22, no.4, p. 1641-1648 (2022), Issue 15306984, 2022, Page(s) 1641-1648, ISSN 1530-6984
Publisher: American Chemical Society
DOI: 10.1021/acs.nanolett.1c04677

Rotavirus Binding to Cell Surface Receptors Directly Recruiting α2 Integrin

Author(s): J. Yang, J. Park, M. Koehler, J. Simpson, Da. Luque, J. M. Rodríguez, D. Alsteens
Published in: Advanced Nanobiomed Research, Issue 26999307, 2021, Page(s) 2100077, ISSN 2699-9307
Publisher: Wiley-VCH GmbH
DOI: 10.1002/anbr.202100077

Molecular insights into receptor binding energetics and neutralization of SARS-CoV-2 variants

Author(s): M. Koehler, A. Ray, R.A. Moreira, B. Juniku, A.B. Poma, D. Alsteens
Published in: Nature Communications, Issue 20411723, 2021, Page(s) 6977, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-27325-1

Altered Glycan Expression on Breast Cancer Cells Facilitates Infection by T3 Seroptype Oncolytic Reovirus

Author(s): D. Mohammed, M. Koehler, A.C. Dumitru, P. Aravamudhan, D.M. Sutherland, T.S. Dermody & David Alsteens
Published in: Nano Letters, Issue 15306984, 2021, Page(s) 9720-9728, ISSN 1530-6984
Publisher: American Chemical Society
DOI: 10.1021/acs.nanolett.1c03608

Multivalent binding of herpesvirus to living cells is tightly regulated during infection

Author(s): Martin Delguste, Caroline Zeippen, Bénédicte Machiels, Jan Mast, Laurent Gillet, David Alsteens
Published in: Science Advances, Issue 4/8, 2018, Page(s) eaat1273, ISSN 2375-2548
Publisher: AAAS
DOI: 10.1126/sciadv.aat1273

Initial Step of Virus Entry: Virion Binding to Cell-Surface Glycans

Author(s): Melanie Koehler, Martin Delguste, Christian Sieben, Laurent Gillet, David Alsteens
Published in: Annual Review of Virology, Issue 7/1, 2020, ISSN 2327-056X
Publisher: Annual Reviews
DOI: 10.1146/annurev-virology-122019-070025

Atomic force microscopy-based mechanobiology

Author(s): Michael Krieg, Gotthold Fläschner, David Alsteens, Benjamin M. Gaub, Wouter H. Roos, Gijs J. L. Wuite, Hermann E. Gaub, Christoph Gerber, Yves F. Dufrêne, Daniel J. Müller
Published in: Nature Reviews Physics, Issue 1/1, 2019, Page(s) 41-57, ISSN 2522-5820
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s42254-018-0001-7

Seeing and sensing single G protein-coupled receptors by atomic force microscopy

Author(s): K Tanuj Sapra, Patrizia M Spoerri, Andreas Engel, David Alsteens, Daniel J Müller
Published in: Current Opinion in Cell Biology, Issue 57, 2019, Page(s) 25-32, ISSN 0955-0674
Publisher: Elsevier BV
DOI: 10.1016/j.ceb.2018.10.006

Glycan-mediated enhancement of reovirus receptor binding

Author(s): Melanie Koehler, Pavithra Aravamudhan, Camila Guzman-Cardozo, Andra C. Dumitru, Jinsung Yang, Serena Gargiulo, Patrice Soumillion, Terence S. Dermody, David Alsteens
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-12411-2

Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166

Author(s): Henri-François Renard, François Tyckaert, Cristina Lo Giudice, Thibault Hirsch, Cesar Augusto Valades-Cruz, Camille Lemaigre, Massiullah Shafaq-Zadah, Christian Wunder, Ruddy Wattiez, Ludger Johannes, Pierre van der Bruggen, David Alsteens, Pierre Morsomme
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-15303-y

Control of Ligand-Binding Specificity Using Photocleavable Linkers in AFM Force Spectroscopy

Author(s): Melanie Koehler, Cristina Lo Giudice, Philipp Vogl, Andreas Ebner, Peter Hinterdorfer, Hermann J. Gruber, David Alsteens
Published in: Nano Letters, Issue 20/5, 2020, Page(s) 4038-4042, ISSN 1530-6984
Publisher: American Chemical Society
DOI: 10.1021/acs.nanolett.0c01426

Molecular interaction and inhibition of SARS-CoV-2 binding to the ACE2 receptor

Author(s): J. Yang, S. J.L. Petitjean, M. Koehler, Q. Zhang, A C. Dumitru, W. Chen, S. Derclaye, S.P. Vincent, P. Soumillion, D. Alsteens
Published in: Nature Communications, Issue 20411723, 2020, Page(s) 4541, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-18319-6

Atomic force microscopy applied to interrogate nanoscale cellular chemistry and supramolecular bond dynamics for biomedical applications

Author(s): Joshua D. Simpson; Ankita Ray; Melanie Koehler; Danahe Mohammed; David Alsteens
Published in: Chemical Communications, Vol. 58, no.33, p. 5072-5087 (2022), Issue 13597345, 2022, Page(s) 5072-5087, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1cc07200e

Multivalent 9-O-Acetylated-sialic acid glycoclusters as potent inhibitors for SARS-CoV-2 infection

Author(s): S. J. L. Petitjean, W. Chen, M. Koehler, R. Jimmidi, J. Yang, D. Mohammed, B. Juniku, M. Stanifer, S. Boulant, S. Vincent, D. Alsteens
Published in: Nature Communications, Issue 20411723, 2022, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-30313-8

Reovirus σ1 Conformational Flexibility Modulates the Efficiency of Host Cell Attachment

Author(s): J.R. Diller, S.R. Halloran, M. Koehler, R. Dos Santos Natividade, D. Alsteens, T. Stehle, T.S. Dermody, K.M. Ogden
Published in: Journal of Virology, Issue 0022538X, 2020, Page(s) e01163-20, ISSN 0022-538X
Publisher: American Society for Microbiology
DOI: 10.1128/jvi.01163-20