We have now successfully completed five field trips – three to Hawaii and two to Bermuda. We have collected samples from over 500 chickens, and have also already obtained the DNA sequences from all 500+ samples, as well as gene expression profiles from a further 1400 samples (comb, bone and the hypothalamus region in the brain). Furthermore, we have sampled three separate Hawaiian islands (Kauai, Oahu, Hawaii), as well as the large number of birds from Bermuda. In addition, we have built an aviary and testing facility on the island of Kauai, and tested over 100 birds in this. These tests have included a variety of different measures of fear and anxiety related behaviour, ranging from predator avoidance behaviour, to socialisation and anxiety responses. In this way, we are now ready to begin the actual analysis to identify genomic regions that have responded to feralisation selection (i.e. the selection that occurs when domestic birds are reintroduced to a natural environment). We will also now map the expression QTL (that is to say the regions that regulate gene expression) in these genomic regions responding to feralisation, as well as throughout the genome. Another important aspect is to compare these feral samples with a controlled wild-domestic lab intercross population based in Sweden. To that end we have performed a methylation-based QTL analysis to identify the genetic elements responsible for the control of local methylation levels in the laboratory population, with this work being published in Nature Ecology and Evolution. We will now be doing a similar analysis in the feral Hawaiian and Bermudian birds. Finally we have also now ordered the reagents required for single cell sequence and epigenome analysis, with this being first conducted in the laboratory population, before we also conduct this on the feral birds.