Integrated control and sensing platform for biopharmaceutical cultivation process high-throughput development and production

Deliverables

Documents, reports (2)

Off-line glycosylation/titre assays after sample purification/preparation on fluidic platform

Different glycan assay formats will be tested for integration into microfluidic platform.

Data-driven models with different complexity levels related to their use (description/soft sensor/control)

Data-driven models will be derived by applying MLPCA to the datasets to determine the data-driven required number of pseudo-bioreactions. In a second step, a decision on the kinetic structure (grey-box vs black-box) will be taken with respect to the data content. Eventually, an adapted identification procedure will be applied and validated with the datasets.

Other (1)

Spectral pre-processing procedures (such as outlier detection, noise reduction and smoothing) are implemented

The algorithms of outlier detection, noise reduction and smoothing are implemented and validated. Furthermore preprocessing procedures like deviation-, distance- and density-based approaches are implemented in the evaluation program.

Publications

Book chapters (1)

High-Throughput Quantification and Glycosylation Analysis of Antibodies Using Bead-Based Assays

Author(s): Sebastian Giehring
Published in: Animal Cell Biotechnology - Methods and Protocols, Issue 2095, 2020, Page(s) 271-284
DOI: 10.1007/978-1-0716-0191-4_15