Objective To understand how the brain works, tools need to be developed to allow the investigation of interactions between individual neurons. Towards this goal, We developed voltage sensing nanoparticles (vsNPs) in the shape of nanorods (vsNRs) that self-insert into the cell membrane and could optically record, non-invasively, action potentials (APs) at the single-particle and nanoscale level, at multiple sites, in a large field-of-view. The proposed nanosensors have unique advantages not offered by other methods: much larger voltage sensitivity, high brightness, and hence single-particle voltage sensitivity, large spectral shift , fast temporal response, minimal photobleaching, large two-photon excitation cross sections, excellent performance in the NIR, and compatibility with lifetime imaging. In this PoC proposal we seek to convert the NVS program from mainly “R” in R&D effort into “D” effort which will allow for IP protection, commercialization, dissemination, and therefore the ‘democratization’ of the technology to the large community of neuroscientists.In order to convert our achievement to commercially viable reagents business for the neuroscience community via the PoC program, we will (i) identify leading applications in neuroscience and brain research, (ii) improve current technology to meet the applications demands by fine tuning the control over size and shape of the particle and testing various types of coating, testing insertion into different lipid compositions in bilayers and cells, and by testing non-specific internalization, (iii) improve the stability and performance reproducibility of the sensors, (iv) develop methods for upscaling the synthesis of these NPs, (v) and formulate GLP protocols required for commercialization.Upon achievement of these goals, we will obtain a versatile, stable, multipurpose, novel tool to be implemented in neuroscience. Fields of science natural sciencesbiological sciencesneurobiologynatural sciencesbiological sciencesbiochemistrybiomoleculeslipidsengineering and technologymaterials engineeringcoating and filmsengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsengineering and technologynanotechnologynano-materials Programme(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Topic(s) ERC-2017-PoC - ERC-Proof of Concept Call for proposal ERC-2017-PoC See other projects for this call Funding Scheme ERC-POC - Proof of Concept Grant Host institution BAR ILAN UNIVERSITY Net EU contribution € 149 566,00 Address BAR ILAN UNIVERSITY CAMPUS 52900 Ramat Gan Israel See on map Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 149 566,00 Beneficiaries (1) Sort alphabetically Sort by Net EU contribution Expand all Collapse all BAR ILAN UNIVERSITY Israel Net EU contribution € 149 566,00 Address BAR ILAN UNIVERSITY CAMPUS 52900 Ramat Gan See on map Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 149 566,00