Nanoscale Biomeasurements of Nerve Cells and Vesicles: Molecular Substructure and the Nature of Exocytosis

Publications

Mass Spectrometry Imaging Shows Modafinil, A Student Study Drug, Changes the Lipid Composition of the Fly Brain.

Author(s): Mai H. Philipsen; Elias Ranjbari; Chaoyi Gu; Andrew G. Ewing
Published in: Angewandte Chemie (International Ed. in English), Issue 2, 2021, Page(s) N/A, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202105004

Nanoscale Amperometry Reveals that Only a Fraction of Vesicular Serotonin Content is Released During Exocytosis from Beta Cells

Author(s): Amir Hatamie, Lin Ren, Haiqiang Dou, Nikhil R. Gandasi, Patrik Rorsman, Andrew Ewing
Published in: Angewandte Chemie International Edition, Issue 60/14, 2021, Page(s) 7593-7596, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202015902

Nanoelectrochemical analysis inside a single living cell

Author(s): Xinwei Zhang, Amir Hatamie, Andrew G. Ewing
Published in: Current Opinion in Electrochemistry, Issue 22, 2020, Page(s) 94-101, ISSN 2451-9103
Publisher: Elsevier
DOI: 10.1016/j.coelec.2020.05.008

Quantifying Intracellular Single Vesicular Catecholamine Concentration with Open Carbon Nanopipettes to Unveil the Effect of L-DOPA on Vesicular Structure.

Author(s): Andrew G. Ewing; Keke Hu; Kim Long Le Vo; Amir Hatamie
Published in: Angewandte Chemie, Issue 1, 2021, Page(s) N/A, ISSN 0044-8249
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/ange.202113406

Localization and Absolute Quantification of Dopamine in Discrete Intravesicular Compartments Using NanoSIMS Imaging

Author(s): Stefania Rabasco; Tho D. K. Nguyen; Chaoyi Gu; Michael E. Kurczy; Nhu T. N. Phan; Andrew G. Ewing
Published in: International Journal of Molecular Sciences; Volume 23; Issue 1; Pages: 160, Issue 1, 2021, Page(s) N/A, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms23010160

Characterization of Stress Granule Protein Turnover in Neuronal Progenitor Cells Using Correlative STED and NanoSIMS Imaging

Author(s): Stefania Rabasco; Alicia A. Lork; Emmanuel Berlin; Tho D. K. Nguyen; Carl Ernst; Nicolas Locker; Andrew G. Ewing; Nhu T. N. Phan
Published in: International Journal of Molecular Sciences, Issue 1, 2023, Page(s) N/A, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24032546

Emerging Data Processing Methods for Single‐Entity Electrochemistry

Author(s): Xinyi Li, Ying‐Huan Fu, Nannan Wei, Ru‐Jia Yu, Huma Bhatti, Limin Zhang, Feng Yan, Fan Xia, Andrew G. Ewing, Yi‐Tao Long, Yi‐Lun Ying
Published in: Angewandte Chemie, Issue 136, 2024, Page(s) e202316551, ISSN 0044-8249
Publisher: John Wiley & Sons
DOI: 10.1002/ange.202316551

Correlating Molecule Count and Release Kinetics with Vesicular Size Using Open Carbon Nanopipettes

Author(s): Keke Hu, Rui Jia, Amir Hatamie, Kim Long Le Vo, Michael V. Mirkin, Andrew G. Ewing
Published in: Journal of the American Chemical Society, Issue 142/40, 2020, Page(s) 16910-16914, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.0c07169

Electrochemical Quantification of Neurotransmitters in Single Live Cell Vesicles Shows Exocytosis is Predominantly Partial

Author(s): Ying Wang, Andrew Ewing
Published in: ChemBioChem, Issue 22/5, 2021, Page(s) 807-813, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000622

Anionic Species Regulate Chemical Storage in Nanometer Vesicles and Amperometrically Detected Exocytotic Dynamics

Author(s): Xiulan He; Andrew G. Ewing
Published in: Journal of the American Chemical Society, Issue 144, 2022, Page(s) 4310-4314, ISSN 1948-7193
Publisher: American Chemical Society
DOI: 10.1021/jacs.2c00581

Chemical Analysis of Single Cells and Organelles

Author(s): Keke Hu, Tho D. K. Nguyen, Stefania Rabasco, Pieter E. Oomen, Andrew G. Ewing
Published in: Analytical Chemistry, Issue 93/1, 2021, Page(s) 41-71, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.0c04361

Single-Vesicle Electrochemistry Following Repetitive Stimulation Reveals a Mechanism for Plasticity Changes with Iron Deficiency.

Author(s): Ying Wang; Chaoyi Gu; Andrew G. Ewing
Published in: Angewandte Chemie International Edition, Issue 4, 2022, Page(s) N/A, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202200716

Hofmeister Series: From Aqueous Solution of Biomolecules to Single Cells and Nanovesicles

Author(s): Xiulan He, Andrew G. Ewing
Published in: ChemBioChem, Issue 24, 2023, Page(s) e202200694, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202200694

Mass Spectrometric Imaging of Plasma Membrane Lipid Alteration Correlated with Amperometrically Measured Activity-Dependent Plasticity in Exocytosis

Author(s): Chaoyi Gu, Mai H. Philipsen, Andrew G. Ewing
Published in: International Journal of Molecular Sciences, Issue 21/24, 2020, Page(s) 9519, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms21249519

Zinc Deficiency Leads to Lipid Changes in Drosophila Brain Similar to Cognitive‐Impairing Drugs: An Imaging Mass Spectrometry Study

Author(s): Mai H. Philipsen, Chaoyi Gu, Andrew G. Ewing
Published in: ChemBioChem, Issue 21/19, 2020, Page(s) 2755-2758, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000197

Electrochemical and Mass Spectrometric Measurement of Enhanced Intravesicular Catecholamine Content and Exocytotic Frequency at Subanaesthetic Ketamine Doses

Author(s): Zhaoying Wang; Xiulan He; Kim Long Le Vo; Andrew G. Ewing
Published in: Analysis & Sensing, Issue 1, 2021, Page(s) 166-170, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anse.202100026

A multimodal electrochemical approach to measure the effect of zinc on vesicular content and exocytosis in a single cell model of ischemia

Author(s): Ying Wang, Chaoyi Gu, Andrew G. Ewing
Published in: QRB Discovery, Issue 2, 2022, Page(s) e12, ISSN 2633-2892
Publisher: Cambridge University Press
DOI: 10.1017/qrd.2021.10

Vesicular release dynamics are altered by the interaction between the chemical cargo and vesicle membrane lipids.

Author(s): Farzaneh Asadpour; Farzaneh Asadpour; Xin-Wei Zhang; Mohammad Mazloum-Ardakani; Meysam Mirzaei; Soodabeh Majdi; Andrew G. Ewing
Published in: Chemical Science, Issue 1, 2021, Page(s) N/A, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1sc02247d

Electrochemical measurements reveal reactive oxygen species in stress granules

Author(s): Keke Hu; Emily Relton; Nicolas Locker; Nhu T. N. Phan; Andrew G. Ewing
Published in: Angewandte Chemie (International Ed. in English), Issue 13, 2021, Page(s) N/A, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202104308

Dysfunction of vesicular storage in young-onset Parkinson's patient-derived dopaminergic neurons and organoids revealed by single cell electrochemical cytometry.

Author(s): Wanying Zhu; Mengdan Tao; Yuan Hong; Shanshan Wu; Chu Chu; Zhilong Zheng; Xiao Han; Qian Zhu; Min Xu; Andrew G. Ewing; Xing Guo; Yan Liu
Published in: Chemical Science, Issue 9, 2022, Page(s) N/A, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d2sc00809b

Dynamic Visualization and Quantification of Single Vesicle Opening and Content by Coupling Vesicle Impact Electrochemical Cytometry with Confocal Microscopy

Author(s): Ying-Ning Zheng; Tho D. K. Nguyen; Johan Dunevall; Nhu T. N. Phan; Andrew G. Ewing
Published in: ACS Measurement Science Au, Issue 1, 2021, Page(s) N/A, ISSN 1948-7193
Publisher: American Chemical Society
DOI: 10.1021/acsmeasuresciau.1c00021

Counteranions in the Stimulation Solution Alter the Dynamics of Exocytosis Consistent with the Hofmeister Series

Author(s): Xiulan He, Andrew G. Ewing
Published in: Journal of the American Chemical Society, Issue 142/29, 2020, Page(s) 12591-12595, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.0c05319

Intracellular injection of phospholipids directly alters exocytosis and the fraction of chemical release in chromaffin cells as measured by nano-electrochemistry

Author(s): Mohaddeseh Aref, Elias Ranjbari, Armaghan Romiani, Andrew G. Ewing
Published in: Chemical Science, Issue 11/43, 2020, Page(s) 11869-11876, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0sc03683h

Concentration of stimulant regulates initial exocytotic molecular plasticity at single cells

Author(s): Xiulan He; Andrew G. Ewing
Published in: Chemical Science, Issue 16, 2022, Page(s) N/A, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1sc05278k

Subcellular Mass Spectrometry Imaging and Absolute Quantitative Analysis across Organelles

Author(s): Aurélien Thomen, Neda Najafinobar, Florent Penen, Emma Kay, Pratik P. Upadhyay, Xianchan Li, Nhu T. N. Phan, Per Malmberg, Magnus Klarqvist, Shalini Andersson, Michael E. Kurczy, Andrew G. Ewing
Published in: ACS Nano, Issue 14/4, 2020, Page(s) 4316-4325, ISSN 1936-0851
Publisher: American Chemical Society
DOI: 10.1021/acsnano.9b09804

Quantitative Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS) Imaging of Individual Vesicles to Investigate the Relation between Fraction of Chemical Release and Vesicle Size

Author(s): Tho Duc Khanh Nguyen, Stefania Rabasco, Alicia A. Lork, Andre Du Toit, Andrew G. Ewing
Published in: Angewandte Chemie International Edition, Issue 62, 2023, Page(s) e202304098, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202304098

Pore-Opening Dynamics of Single Nanometer Biovesicles at an Electrified Interface.

Author(s): Xinwei Zhang; Andrew G. Ewing
Published in: ACS Nano, Issue 16, 2022, Page(s) 9852-9858, ISSN 1948-7193
Publisher: American Chemical Society
DOI: 10.1021/acsnano.2c03929

Amperometry and Electron Microscopy show Stress Granules Induce Homotypic Fusion of Catecholamine Vesicles

Author(s): Hui Gu, Chaoyi Gu, Nicolas Locker, Andrew G. Ewing
Published in: Angewandte Chemie, Issue 136, 2024, Page(s) e202400422, ISSN 0044-8249
Publisher: John Wiley & Sons
DOI: 10.1002/ange.202400422

Single Exosome Amperometric Measurements Reveal Encapsulation of Chemical Messengers for Intercellular Communication

Author(s): Keke Hu, Kim Long Le Vo, Fan Wang, Xin Zhang, Chaoyi Gu, Ning Fang, Nhu T. N. Phan, Andrew G. Ewing
Published in: Journal of the American Chemical Society, Issue 145, 2023, Page(s) 11499-11503, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.3c02844

Vesicle Impact Electrochemical Cytometry to Determine Carbon Nanotube-Induced Fusion of Intracellular Vesicles.

Author(s): Amir Hatamie; Lin Ren; Xin-Wei Zhang; Andrew G. Ewing
Published in: Analytical Chemistry, Issue 93, 2021, Page(s) 13161-13168, ISSN 1948-7193
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.1c01462

Anticancer Drug Tamoxifen Affects Catecholamine Transmitter Release and Storage from Single Cells

Author(s): Zahra Taleat, Anna Larsson, Andrew G. Ewing
Published in: ACS Chemical Neuroscience, Issue 10/4, 2019, Page(s) 2060-2069, ISSN 1948-7193
Publisher: American Chemical Society
DOI: 10.1021/acschemneuro.8b00714

Dopamine Release Dynamics in the Tuberoinfundibular Dopamine System

Author(s): Stefanos Stagkourakis, Johan Dunevall, Zahra Taleat, Andrew G. Ewing, Christian Broberger
Published in: The Journal of Neuroscience, Issue 39/21, 2019, Page(s) 4009-4022, ISSN 0270-6474
Publisher: Society for Neuroscience
DOI: 10.1523/jneurosci.2339-18.2019

Extracellular ATP Regulates the Vesicular Pore Opening in Chromaffin Cells and Increases the Fraction Released During Individual Exocytosis Events

Author(s): Soodabeh Majdi, Anna Larsson, Neda Najafinobar, Ricardo Borges, Andrew G. Ewing
Published in: ACS Chemical Neuroscience, Issue 10/5, 2019, Page(s) 2459-2466, ISSN 1948-7193
Publisher: American Chemical Society
DOI: 10.1021/acschemneuro.8b00722

Combined Amperometry and Electrochemical Cytometry Reveal Differential Effects of Cocaine and Methylphenidate on Exocytosis and the Fraction of Chemical Release

Author(s): Wanying Zhu, Chaoyi Gu, Johan Dunevall, Lin Ren, Xuemin Zhou, Andrew G. Ewing
Published in: Angewandte Chemie, Issue 131/13, 2019, Page(s) 4282-4286, ISSN 0044-8249
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/ange.201813717

Interplay between Cocaine, Drug Removal, and Methylphenidate Reversal on Phospholipid Alterations in Drosophila Brain Determined by Imaging Mass Spectrometry

Author(s): Mai Hoang Philipsen, Nhu T. N. Phan, John Stephen Fletcher, Andrew G. Ewing
Published in: ACS Chemical Neuroscience, Issue 11/5, 2020, Page(s) 806-813, ISSN 1948-7193
Publisher: American Chemical Society
DOI: 10.1021/acschemneuro.0c00014

Spatial Lipidomics Reveals Region and Long Chain Base Specific Accumulations of Monosialogangliosides in Amyloid Plaques in Familial Alzheimer’s Disease Mice (5xFAD) Brain

Author(s): Ibrahim Kaya, Eva Jennische, Johan Dunevall, Stefan Lange, Andrew G. Ewing, Per Malmberg, Ahmet Tarik Baykal, John S. Fletcher
Published in: ACS Chemical Neuroscience, Issue 11/1, 2020, Page(s) 14-24, ISSN 1948-7193
Publisher: American Chemical Society
DOI: 10.1021/acschemneuro.9b00532

Mass spectrometry imaging as a novel approach to measure hippocampal zinc

Author(s): Masoumeh Dowlatshahi Pour, Lin Ren, Eva Jennische, Stefan Lange, Andrew G. Ewing, Per Malmberg
Published in: Journal of Analytical Atomic Spectrometry, Issue 34/8, 2019, Page(s) 1581-1587, ISSN 0267-9477
Publisher: Royal Society of Chemistry
DOI: 10.1039/c9ja00199a

Vesicular Transmitter Content in Chromaffin Cells Can Be Regulated via Extracellular ATP

Author(s): Anna Larsson, Soodabeh Majdi, Ricardo Borges, Andrew Ewing
Published in: ACS Chemical Neuroscience, Issue 10/11, 2019, Page(s) 4735-4740, ISSN 1948-7193
Publisher: American Chemical Society
DOI: 10.1021/acschemneuro.9b00494

Altered Lipid Composition of Secretory Cells Following Exposure to Zinc Can Be Correlated to Changes in Exocytosis

Author(s): Lin Ren, Masoumeh Dowlatshahi Pour, Per Malmberg, Andrew G. Ewing
Published in: Chemistry – A European Journal, Issue 25/21, 2019, Page(s) 5406-5411, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201900010

Intracellular Electrochemical Nanomeasurements Reveal that Exocytosis of Molecules at Living Neurons is Subquantal and Complex

Author(s): Anna Larsson, Soodabeh Majdi, Alexander Oleinick, Irina Svir, Johan Dunevall, Christian Amatore, Andrew G. Ewing
Published in: Angewandte Chemie International Edition, 2020, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201914564

Plasticity in exocytosis revealed through the effects of repetitive stimuli affect the content of nanometer vesicles and the fraction of transmitter released

Author(s): Chaoyi Gu, Anna Larsson, Andrew G. Ewing
Published in: Proceedings of the National Academy of Sciences, Issue 116/43, 2019, Page(s) 21409-21415, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1910859116

Using Single-Cell Amperometry and Intracellular Vesicle Impact Electrochemical Cytometry To Shed Light on the Biphasic Effects of Lidocaine on Exocytosis

Author(s): Daixin Ye, Chaoyi Gu, Andrew Ewing
Published in: ACS Chemical Neuroscience, Issue 9/12, 2018, Page(s) 2941-2947, ISSN 1948-7193
Publisher: American Chemical Society
DOI: 10.1021/acschemneuro.8b00130

Amperometric Measurements and Dynamic Models Reveal a Mechanism for How Zinc Alters Neurotransmitter Release

Author(s): Lin Ren, Alexander Oleinick, Irina Svir, Christian Amatore, Andrew G. Ewing
Published in: Angewandte Chemie International Edition, Issue 59/8, 2020, Page(s) 3083-3087, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201913184

On-Tissue Chemical Derivatization of Catecholamines Using 4-( N -Methyl)pyridinium Boronic Acid for ToF-SIMS and LDI-ToF Mass Spectrometry Imaging

Author(s): Ibrahim Kaya, Steffen M. Brülls, Johan Dunevall, Eva Jennische, Stefan Lange, Jerker Mårtensson, Andrew G. Ewing, Per Malmberg, John S. Fletcher
Published in: Analytical Chemistry, Issue 90/22, 2018, Page(s) 13580-13590, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.8b03746

Simultaneous Quantification of Vesicle Size and Catecholamine Content by Resistive Pulses in Nanopores and Vesicle Impact Electrochemical Cytometry

Author(s): Xin-Wei Zhang, Amir Hatamie, Andrew G. Ewing
Published in: Journal of the American Chemical Society, Issue 142/9, 2020, Page(s) 4093-4097, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.9b13221

Nano-analysis Reveals High Fraction of Serotonin Release during Exocytosis from a Gut Epithelium Model Cell.

Author(s): Ying Wang; Chaoyi Gu; Bhavik Anil Patel; Andrew G. Ewing
Published in: Angewandte Chemie (International Ed. in English), Issue 7, 2021, Page(s) N/A, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/ange.202108193

Direct Acquisition of the Gap Height of Biological Tissue-Electronic Chemical Sensor Interfaces.

Author(s): Xin‐Wei Zhang; Amir Hatamie; Andrew G. Ewing
Published in: Angewandte Chemie, Issue 1, 2022, Page(s) N/A, ISSN 0044-8249
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202210224

Comparison of Disk and Nanotip Electrodes for Measurement of Single-Cell Amperometry during Exocytotic Release

Author(s): Chaoyi Gu, Xinwei Zhang, Andrew G. Ewing
Published in: Analytical Chemistry, Issue 92/15, 2020, Page(s) 10268-10273, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.0c02013

Potentiometric pH Nanosensor for Intracellular Measurements: Real-Time and Continuous Assessment of Local Gradients.

Author(s): Mohaddeseh Aref; Elias Ranjbari; Juan José García-Guzmán; Keke Hu; Alicia Lork; Gastón A. Crespo; Andrew G. Ewing; Maria Cuartero
Published in: Analytical Chemistry, Issue 93, 2021, Page(s) 15744-15751, ISSN 1948-7193
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.1c03874

Simultaneous detection of vesicular content and exocytotic release with two electrodes in and at a single cell

Author(s): Chaoyi Gu; Andrew G. Ewing
Published in: Chemical Science, Issue 3, 2021, Page(s) N/A, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d1sc01190a

Direct Measurement of Total Vesicular Catecholamine Content with Electrochemical Microwell Arrays

Author(s): Elias Ranjbari, Zahra Taleat, Mokhtar Mapar, Mohaddeseh Aref, Johan Dunevall, Andrew Ewing
Published in: Analytical Chemistry, Issue 92/16, 2020, Page(s) 11325-11331, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.0c02010

Simultaneous Counting of Molecules in the Halo and Dense‐Core of Nanovesicles by Regulating Dynamics of Vesicle Opening

Author(s): Xiulan He; Andrew G. Ewing
Published in: Angewandte Chemie International Edition, Issue 12, 2022, Page(s) N/A, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/ange.202116217

Omega-3 and -6 Fatty Acids Alter the Membrane Lipid Composition and Vesicle Size to Regulate Exocytosis and Storage of Catecholamines

Author(s): Chaoyi Gu, Mai H. Philipsen, Andrew G. Ewing
Published in: ACS Chemical Neuroscience, Issue 15, 2024, Page(s) 816-826, ISSN 1948-7193
Publisher: American Chemical Society
DOI: 10.1021/acschemneuro.3c00741

Visualization of Partial Exocytotic Content Release and Chemical Transport into Nanovesicles in Cells.

Author(s): Tho Duc Khanh Nguyen; Lisa Mellander; Alicia Lork; Aurélien Thomen; Mai Philipsen; Michael E. Kurczy; Nhu T.N. Phan; Andrew G. Ewing
Published in: ACS Nano, Issue 16, 2022, Page(s) 4831-4842, ISSN 1948-7193
Publisher: American Chemical Society
DOI: 10.1021/acsnano.2c00344

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